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- Cells were incubated with chloroquine for 2 hours before stimulation with lipopolysaccharide (LPS, lg/ml). Cell viability was measured by staining cells with 3-(4,5-dimethylthiazol-2- yl)-2,5- diphenyltetrazolium bromide. TNF in the culture supernatant and the cell lysate were measured by sandwich ELISA, using specific monoclonal antibodies according to the manufacturer's instructions. To determine the level of cell-associated cytokines, cells were washed with phosphate-buffered saline (PBS) and lysed in a buffer containing 0.5% NP-40. Standard recombinant proteins were also diluted in PBS containing the same concentrations of NP-40 as the cell lysate. Cytokine synthesized were proteolytically cleaved and released into medium. The amount of cytokines in the culture supernatant and the cell lysate were presented. Results for TNF production are shown in Figure 1 (Jang et al, 2006). (Cells : PBMC: Human peripheral blood mononuclear cells, monocytes, monocytic lymphoma U-937 and leukemia…Differentiate Widal from Weil-Felix test. The strains of Proteus vulgaris are used in Weil-felix reaction. Explain why.I AM TRYING TO IDENTIFY THIS UNKNOWN. IMAGE 1 HAS TWO PICTURE OF CATALASE TEST AND BLOOD AGAR TEST. I believe it is one of the following: 1) S. pyo. 2)S. agal . 3)S.pneu. 4)E. faecalis 5)S. aureus 6)S epi. 7)S. sapro. 8)M. luteus Please let me know which test will i need out of the table to justify your reason of picking up the unknown and also how that test justifies it? what characteristics of that test made you pick the unknown?
- List the following reagents in the order in which they are added to an ELISA: secondary antibody, bodily fluid sample, enzyme substrate, primary antibody O Scondary antibody, bodily fluid sample, primary antibody, enzyme substrate O Enzyme substrate, primary antibody, secondary antibody, bodily fluid sample Bodily fluid sample, enzyme substrate, secondary antibody, primary antibody Bodily fluid sample, primary antibody, secondary antibody, enzyme substrateCells were incubated with chloroquine for 2 hours before stimulation with lipopolysaccharide (LPS, 1g/ml). Cell viability was measured by staining cells with 3-(4,5-dimethylthiazol-2- yl)-2,5- diphenyltetrazolium bromide. TNF in the culture supernatant and the cell lysate were measured by sandwich ELISA, using specific monoclonal antibodies according to the manufacturer's instructions. To determine the level of cell-associated cytokines, cells were washed with phosphate-buffered saline (PBS) and lysed in a buffer containing 0.5% NP-40. Standard recombinant proteins were also diluted in PBS containing the same concentrations of NP-40 as the cell lysate. Cytokine synthesized were proteolytically cleaved and released into medium. The amount of cytokines in the culture supernatant and the cell lysate were presented. Results for TNF production are shown in Figure1 (Jang et al, 2006). (Cells : PBMC: Human peripheral blood mononuclear cells, monocytes, monocytic lymphoma U-937 and leukemia…What is the chemical basis of the Xanthoproteic Test? Give the chemical reaction involved.
- I AM TRYING TO IDENTIFY THIS UNKNOWN. ***IMAGE 1 HAS TWO PICTURE OF CATALASE TEST AND BLOOD AGAR TEST. ***IMAGE 2 HAS TWO TESTS CONDUCTED ON IT, BACITRACIN AND PYR I believe it is one of the following: 1) S. pyo. 2)S. agal . 3)S.pneu. 4)E. faecalis 5)S. aureus 6)S epi. 7)S. sapro. 8)M. luteus Please let me know which one of the above is thge unknown. You have pictures of the unkown on Catalase and Blood Agar and three test conducted on Bacitracin and PYR. Give reasoning as why you think it will be one of the above. Explaing the characteristics and what made you deicide it?Answer the following questions based on Kirby Bauer / MacFarland standards 1-0.5 McFarland standard solution is ideally used for inoculation of plated for Kirby Bauer test. a)True b)False Principle of Kirby Bauer test is the diffusion of antibiotic agents in to Agar based media. a) True b) FalseName three reasons why the ELISA test is useful in detecting microbes