Mutation analysis of GCK gene in patients with diabetes revealed a c.114 T→A (shown in bold andunderlined) substitution in heterozygote state. In order to check the mutation in healthy individuals,restriction enzyme analysis will be used.a) Which enzyme can we use to differentiate wild type and mutant sequence? Please indicate whichallele (wild type or mutant allele) will be cut with the restriction enzyme. Use table 1 shown below.b) Draw the expected agarose gel result of a homozygous wild type, homozygous mutant andheterozygote individual after restriction enzyme analysis.ATGAGGCTCTTTGCCACCAGTCCCAGTTTTATGCATGGCAGCTCTAATGACAGGATGGTCACCCCTGCTGAGGCCACTCCTGGTCACCATGACAACCACAGGCCCTCTCAGTATCACAGTAAGCCCTGGCAGGAGAATCCCCCACTCCACACCTGGCTGGAGCACGAAATGCCGAGCGGCGCCTGAGCCCCAGGGAAGCAGGCTAGGATGTGAFigure 1. GCK gene sequence. Length of the fragment is 213bp.Table1. The restriction enzymes and their recognition sequences.Restriction enzymeRecognition seguencewwwwwwtw wwNar IGG/CGCCDde IC/TNAGHae IINGCGC/nHpallCc/GGAlulAG/CTSmalCCC/GGGMbol/GATCMae III/GTNACBsp 1286 IGnGCn/CHind IIA/AGCTTEcoR IG/AATTCn: any nucleotide/: cutting site田

Name: Mutation analysis of GCK gene in patients with diabetes revealed a c.
Uploaded: 2024-03-20T06:42:55.000Z
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Description: Mutation analysis of GCK gene in patients with diabetes revealed a c.114 T→A (shown in bold andunderlined) substitution in heterozygote state. In order to check the mutation in healthy individuals,restriction enzyme analysis will be used.a) Which en