Consider the λ bacteriophage DNA molecule as shown in Figure 21.14. Total digestion with the two restriction endonucleases used does not allow unambiguous placement of restriction sites, as shown in the map. Describe restriction patterns—either from partial digestion or from digestion with both BamHI and EcoRI—that would help to establish the specific map of restriction sites shown in Figure 21.14(b). FragmentFragmentsize, kbECORI BamHIFragmentsize, kbFragmentdigest digestA'21.2-16.8B-12.1F/AD'7.4-7.2E5.8-6.8 + 6.5-5.6+ 5.5D, FА, С5.6-4.9B'3.5Fragments with verysimilar sizes form butone band on a gel.(a) Experimental determination of fragmentation pattems, resulting from enzymaticdigestion of the 48.5-kb linear DNA molecule from phage A. Restriction digests aresubjected to agarose gel electrophoresis, and the fragments are visualized underultraviolet light after staining the gel with ethidium bromide, a fluorescent dye.• FIGURE 21.14 Fragmentation of bacteriophage A DNA withrestriction endonucleases EcoRI or BamHI. Mapping the restric-tion sites also requires data from partial digestion or with digestionof the DNA with EcoRI and BamHI together (not shown).BamHI sitesA DNABamHI segmentsABEEcoRI sitesA DNAEcoRI segmentsA'B'C'D'E'E'1020304048.5DNA length, kb(b) Restriction maps of cleavage sites for each enzyme on the DNA molecule. Byconvention, the fragments are assigned letters as shown. In this experiment the12.1-kb fragment in the BamHI digest resulted from linking the A and F (terminal)fragments by base pairing at their cohesive ends (Chapter 23).

DNA or deoxyribonucleic acid is a type of biomolecule. It is a polymer of deoxyribonucleotides…

Answered: Fragment Fragment size, kb ECORI BamHI…

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Chapter5: Nutrition, Attitudes, And Behavior

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Consider the λ bacteriophage DNA molecule as shown in Figure 21.14. Total digestion with the two restriction endonucleases used does not allow unambiguous placement of restriction sites, as shown in the map. Describe restriction patterns—either from partial digestion or from digestion with both BamHI and EcoRI—that would help to establish the specific map of restriction sites shown in Figure 21.14(b).

Fragment
Fragment
size, kb
ECORI BamHI
Fragment
size, kb
Fragment
digest digest
A'
21.2
-16.8
B
-12.1
F/A
D'
7.4
-7.2
E
5.8-
6.8 + 6.5
-5.6+ 5.5
D, F
А, С
5.6-
4.9
B'
3.5
Fragments with very
similar sizes form but
one band on a gel.
(a) Experimental determination of fragmentation pattems, resulting from enzymatic
digestion of the 48.5-kb linear DNA molecule from phage A. Restriction digests are
subjected to agarose gel electrophoresis, and the fragments are visualized under
ultraviolet light after staining the gel with ethidium bromide, a fluorescent dye.
• FIGURE 21.14 Fragmentation of bacteriophage A DNA with
restriction endonucleases EcoRI or BamHI. Mapping the restric-
tion sites also requires data from partial digestion or with digestion
of the DNA with EcoRI and BamHI together (not shown).
BamHI sites
A DNA
BamHI segments
A
B
E
EcoRI sites
A DNA
EcoRI segments
A'
B'
C'
D'
E'
E'
10
20
30
40
48.5
DNA length, kb
(b) Restriction maps of cleavage sites for each enzyme on the DNA molecule. By
convention, the fragments are assigned letters as shown. In this experiment the
12.1-kb fragment in the BamHI digest resulted from linking the A and F (terminal)
fragments by base pairing at their cohesive ends (Chapter 23).

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