Metabolizing a fatty acid via the B-oxidation pathway will generate acetyl CoA, NADH and FADH2. Based on your biochemical knowledge, the major metabolic intermediate will enter cellular respiration in [Select] , and the reduced coenzymes will enter cellular respiration in [Select] Could a mature red blood cell perform the B-oxidation pathway? Yes, in their cytoplasm.
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- Ethanol as a Source of Metabolic Energy (Integrates with Chapters 19 and 20.) Acetate produced in ethanol metabolism can be transformed into acetyl-COA by the acetyl thiokinase reaction: Acetate+ATP+CoASHacetyleCoA+AMP+PPiAcetyle-CoA then can enter the citric acid cycle and undergo oxidation to 2 CO2by this route, assuming oxidative phosphorylation is part of the process? (Assume all reactions prior to acetyl-CoA entering the citric acid cycle occur outside the mitochondrion). Per carbon atom, which is a better metabolic fuel, ethanol or glucose? That is, how many ATP equivalents per carbon atom are generated by combustion of glucose versus ethanol to CO2?Understanding the Mechanism of Hemolytic Anemia Genetic defects in glycolytic enzymes can have serious consequences for humans For example, defects in the gene for pyruvate kinase can result in a condition known as hemolytic anemia. Consult a reference to learn about hemolytic anemia, and discuss why such genetic defects lead to this condition.Using the ActiveModel for enoyl-CoA dehydratase, give an example of a case in which conserved residues in slightly different positions can change the catalytic rate of reaction.
- Extending the Mechanism of Methylmalonyl-CoA Mutase to Similar Reactions Based on the mechanism for the methylmalonyl-CoA mutase (see problem 14), write reasonable mechanisms for the following reactions shown.In the complete b-oxidation of stearic acid (18:0), a) how many moles of acetyl CoA are produced? b) how many times would the fatty acid pass through the fatty acid spiral for complete oxidation? c) what is the total ATP produced in the citric acid cycle? d) how many moles of NADH are produced? e) how many moles of FADH2 are produced? f) how many moles of ATP are produced after all NADH are reoxidized in the respiratory chain? g) how many moles of ATP are produced after all FADH2 are reoxidized in the respiratory chain? h) how many moles of ATP are produced from the complete oxidation of stearic acid?In the complete b-oxidation of stearic acid (18:0), a)how many moles of acetyl CoA are produced? ________ b)how many times would the fatty acid pass through the fatty acid spiral for complete oxidation? ________ c)what is the total ATP produced in the citric acid cycle? ________ d)how many moles of NADH are produced? ________ e)how many moles of FADH2 are produced? ________ f)how many moles of ATP are produced after all NADH are reoxidized in the respiratory chain? ________ g)how many moles of ATP are produced after all FADH2 are…
- In response low density lipoprotein (LDL), cells elevate the activity of ATP-citrate lyase, which catalyzes the conversion of citrate to acetyl-CoA and oxaloacetate. TRUE or FALSE?Fatty acids are oxidized by acyl COA dehydrogenase. Branched fatty acids, containing a methyl group at beta carbons cannot be oxidized by this enzyme. How does the oxidation of such branched fatty acids take place? Explain with all set of reactions and enzymes involvedThe total degradation of a fatty acid with an odd number of carbons yields acetyl-CoA and another compound, X. Show the structure of X, and describe the pathway by which it is converted into a citric acid cycle intermediate, including where any cofactors participate.
- Synthesis of the activated form of acetate (acetyl-CoA) is carried out in an ATP-dependent process:(a) The ΔG′° for hydrolysis of acetyl-CoA to acetate and CoA is −32.2 kJ/mol and that for hydrolysis of ATP to AMP and PPi is −30.5 kJ/mol. Calculate ΔG′° for the ATPdependent synthesis of acetyl-CoA.(b) Almost all cells contain the enzyme inorganic pyrophosphatase, which catalyzes the hydrolysis of PPi to Pi. What effect does the presence of this enzyme have on the synthesis of acetyl-CoA? Explain.Fatty acid oxidation for energy yield occurs in the mitochondrial matrix, and for palmitate as one example, generates [FADN2, NADH, and Acetyle-CoA or FADH, GTP, and NADPH] , all of which can be converted to ATP (108 of them to be exact) via oxidative phosphorylation. This is a [greater or smaller] yield of ATP per carbon atom compared to glucose. Ketogenesis is a process where acetyl-CoA (including that from breakdown of fatty acids) is converted to ketone bodies under conditions where carbohydrates are [in excess or liminting] . Acetoacetate and D-beta-hydroxybutyrate are delivered from liver to the blood stream where they provide energy for [cardiac and skeletal muscle as well as brain or synthesis of glycogen] . [Insulin or glucagon] promotes ketogenesis by stimulating fatty acid export from adipose tissue.The same reactions that were seen in the process of converting pyruvate to acetyl-S-CoA, occur in step ____ of the citric acid cycle as ____________ is converted to _________________.