2) A scientist wants to increase the amount of sample of a DNA sequence of 100 bps. Calculate the number cycles of PCR required to generate 1 ng of DNA, when starting from a single copy. Note that the molar mass of an average DNA bp is 600 g/mol.

Q: You are using a spectrophotometer to determine the concentration of DNA in a PCR product, but your…

A: The concentration of DNA and RNA should be determined by measuring the absorbance at 260 nm (A260)…

Q: An analyst designed a pair of primers to detect the presence of Alu sequence in human DNA genome…

A: PCR stands for a polymerase chain reaction. PCR is carried out under in-vitro or test tube…

Q: 5) PCR primers have a melting temperature (Tm) at which they become dissociated from the template…

A: A primer is a short strand of nucleotide molecules that serves as an initial point for the synthesis…

Q: If you design a set of primers for a PCR reaction, please describe the temperature that you will set…

A: PCR This is Polymerase Chain Reaction. This technique is used to amplify the specific sequences of…

Q: PCR is a very useful method in biotechnology because it does not require the 6 or more different key…

A: PCR is a polymerase chain reaction- it ia process of replication of DNA in laboratory. PCR can…

Q: You are an expert molecular biologist and you just had your regular PCR run and you are now checking…

A: Following are the steps you will make to be able to avoid this in your next PCR run?   1. do the…

Q: During a PCR reaction, the step in the cycle where dNTPs are added to the primer 3’ ends to fill in…

A: Kary Mullis created the groundbreaking technique known as PCR (Polymerase Chain Reaction) in the…

Q: What size would the PCR product be for a different chromosome where there were nine tandem repeats.

A: The each STR is 5 bp. So, in the given diagram the total length of the PCR product is 420 bp. It…

Q: What would be the expected effect on the PCR reaction, if you increased the temperature of the…

A: The polymerase chain reaction (PCR) is a technique for amplifying DNA sequences in the laboratory.…

Q: Determine the chemical reagents utilized in banana DNA extraction and their roles. What role does…

A: 3. Liquid dishwashing soap This helps split apart the cell membrane made of lipids and the cell…

Q: You want to set up 50 µl total volume of a PCR reaction. You have a microfuge tube of Taq polymerase…

A:

Q: In a PCR reaction to amply a particular gene, 120 copy of the gene were initially used as DNA…

A: Here, option A is the right answer. You may use the following formula to calculate the number of…

Q: Protocols in isolating DNA often involve the use of two kinds of ethanol, 100% ethanol and  70%…

A: 70% ethanol is used in washing DNA furing DNA extraction. 100% ethanol is used in the final step. In…

Q: If a sample of double-stranded DNA gave the following results, what is the concentration (ng/uL) of…

A: The absorption value of 1 at A260 corresponds to 50 ug/ml So, the value of 1.637 at A260 corresponds…

Q: There are many types of PCR techniques available nowadays. Explain the concept of nested R and…

A: PCR stands for polymerase Chain Reaction. It is a technique which is used to amplify a given gene…

Q: Explain the importance of different temperatures in PCR. Tm of forward primer= 62 °C Tm of reverse…

A: Polymerase chain reactions are the technique used to make millions of copies rapidly of a DNA…

Q: The exponential nature of PCR allows spectacular increases in the abun- dance of a DNA sequence…

A: PCR (polymerase chain reaction is a technique through which a DNA segment is copied to a million…

Q: Houra put together a PCR reaction with the following ingredients: 5 uL of 10X buffer with Mg+2 2.5…

A: Polymerase Chain Reaction --( PCR ) --  DNA of various origin are not directly analyze , it is very…

Q: What are the reasons why there needs to be more than 10 cycles in the PCR process? Name 3 reasons.

A: DNA sequences can be amplified in vitro using the PCR (Polymerase Chain Reaction) method. A…

Q: List and briefly describe the function of 4 proteins used in DNA replication in a E. coli but NOT…

A: The process of copying a DNA molecule to produce its two identical copies is termed as the DNA…

Q: A student carries out PCR using the following steps:  step 1: 94C for 1 minute Step 2: 60C for…

A: A complete set of chromosomes in any species is regarded as its genome. Each  chromosomes homes DNA…

Q: What is expected theoretical number of copies of DNA molecules after 28 cycles in a PCR experiment?…

A: INTRODUCTION: DNA : Its fullform is Deoxyribo nucleic acid and It has a double stranded helix…

Q: Each PCR cycle has three steps: DNA sample denaturation, primer annealing, and elongation/extension…

A: Polymerase chain reaction (PCR) is a simple technique to amplify a DNA template to produce specific…

Q: You are working in a molecular biology laboratory and are having challenges with your PCR. You…

A: Polymerase chain reaction (PCR) is the method employed by the geneticist to multiply the number or…

Q: Polymerase Chain Reaction (PCR) works by exposing the reaction to a series of temperature changes.…

A: Polymerase chain reaction (PCR) is a scientific method that exponentially multiplies a single copy…

Q: Most PCR reactions do not use the more expensive types of DNA polymerase, which have DNA…

A: DNA polymerases inside the cell, especially the polymerases I, II, and III, have proof reading…

Q: Please answer these two questions regarding PCR:   a) Why do you need to perform PCR on DNA obtained…

A: The polymerase chain reaction (PCR) is a technique used to make many copies of a specific segment of…

Q: During PCR, the reaction mixture cycles through three temperatures (for example 94, 60, and 72…

A: Introduction :  Using the thermo-resistant DNA polymerase, the polymerase chain reaction, also known…

Q: Lane M 1 2 3 4 5 Content DNA ladder Replicate 1 Replicate 2 Replicate 3 Negative control Positive…

A: PCR stands for polymerase chain reaction. PCR is used to amplify nucleic acid segments for further…

Q: Component 1 PCR buffer 2 MgCl2 3 dNTP mix 4 SSR forward primer 5 SSR reverse primer 6 Taq polymerase…

A: PCR is also known as a polymerase chain reaction which is generally used to amplify the template DNA…

Q: Above are the results of gel electrophoresis following digestion with restriction enzymes. What is…

A: Using specialized enzymes known as restriction endonucleases, restriction digestion is a laboratory…

Q: please solve this with step-by-step calculations and explanations.

A: 1:

Q: STR #1: D5S818 allele 1: 5'-CAATCATAGCCACAA | GATAGATAGATAGATAGATAGATAGATACCAAAGAG-3' allele 2:…

A: DNA profiling is a laboratory forensic technique used to obtain the specific pattern of DNA from a…

Q: PCR is quick, efficient and easy to perform. However, there are some situations when cell-based…

A: PCR which stands for polymerization chain reaction is an analytic technique which is used to amplify…

Q: PCR primers are designed to only replicate the N gene sequence of the viral genome. Part of the N…

A: Polymerase chain reaction, or PCR, is a molecular biology technique to make many copies of a…

Q: What are the reasons why there needs to be more than 10 cycles in the PCR process? Name 2 reasons.

A: PCR (Polymerase Chain Reaction) is a technique used to amplify DNA sequences in vitro. The process…

Q: PCR is a powerful technique to screen and amplify segments of DNA for use in recombinant protein…

A: Introduction PCR is a method for amplifying a specific region of DNA from a mixed template. Reagents…

Q: ou are an expert molecular biologist and you just had your regular PCR run and you are now checking…

A: Polymerase Chain Reaction PCR is an effective technique for producing large quantities of specific…

Q: Ideal primers for the PCR reaction should have the following feature: they should have a high A-T…

A: Polymerase chain reaction (PCR) is a laboratory technique for rapidly producing (amplifying) large…

Q: You want to set up 50 µl total volume of a PCR reaction. You have a microfuge tube of Forward primer…

A: Polymerase chain reaction (PCR) is a widely used method for rapidly producing millions to billions…

Q: Which of the following chemicals or procedures is not involved in a typical Polymerase Chain…

A: Introduction :- PCR (polymerase chain reaction) is a laboratory technique used to amplify a specific…

Q: Polymerase Chain Reaction (PCR) was invented by Kary Mullis in 1983. This technique had indeed…

A: In molecular biology, PCR (Polymerase Chain Reaction) is a common tool which is used in making…

Q: In lane 1, a size standard was loaded, which contained a mix a DNA fragments known to be 1000 bp,…

A: Introduction Comparison between the location and size of the standard and human samples dictates the…

Q: The current state-of-the-art in forensic DNA profiling involves the PCR-amplification and analysis…

A: PCR is a process in which millions of copies of a specific sequence of DNA can be made in a matter…

Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.