Malin just discovered a new protein, Malinase and for it not to unfold and lose its function, he realized that the protein needs to be constantly immersed in a pH = 11.90 solution. He is therefore interested in preparing a 50.0 mL 0.080 M pH = 11.90 buffer to contain the protein. He listed down the acid solutions available to his disposal, and also looked for their acid dissociation constant (Ka) values. ACID Ка Каз Кa N/A N/A 4.00 x 10* 2.00 M NITROUS ACID (HNO:) 2.00 M ACETIC ACID (CH,COOH) 2.00 M ASCORBIC ACID (H;Asc) 2.00 M PHOSPHORIC ACID (H;POA) 7.52 x 10 6.23 x 10 4.28 x 103 N/A 1.76 x 10 N/A 7.90 x 10 1.60 x 102 N/A A. Identify which weak acid system is best to use in preparing the buffer? Why? В. Identify the species which will make up the buffer system? C. prepare the buffer Calculate the amount (in mmoles) of each of the components necessary to D. Indicate how Malin is supposed to prepare the buffer from 2.00 M of the acid and/or 4.00 M N2OH. E. Calculate the resulting pH if 5 drops (1 ml = 20 drops) 0.500 M HCI was accidentally added to the buffer.

Determine the isoelectric point of glutamic acid.(a) Draw the chemical structures of the amino acid in the pH ranges that you will use for the determination of pl.(b) Show all calculations for determining the pl of glutamic acid.

All amino acids have two ionizable functional groups: an α‑amino group (average pKa of 9.4) and an α‑carboxylic acid group (average pKa of 2.2). Aspartic acid has an ionizable side chain (R group) with a pKa of about 3.8. One of the possible ionization states of aspartic acid is shown in the image. At what pH would the structure be the predominant ionization state? Consider the ionization state of all three of the functional groups.

What would the structure and net charge of y- Aminobutyric acid (GABA) be at pH = 1?

Differentiate and explain the chemical basis of the Kjeldahl, Lowry and Bradford techniques that can be used to quantitate proteins in quality control and research.

The isoelectric point (pl) of phenylalanine is pH 5.5. Draw the structure of the major form of glutamic acid at pH values of (a) 1 (b) 5.5 (c) 11.0.

The pK₂ of acetic acid, HC₂H3O2, is 4.96. A buffer solution was made using an unspecified amount of acetic acid and 0.1 moles of NaC2H3O2 in enough water to make 1.82 liters of solution. Its pH was measured as 4.04. How many moles of HC2H3O2 were used? Report your answer with 2 places past the decimal point. Do not put unit in your answer.

How does the shape of a titration curve confirm the fact that the pH region of greatest buffering power for an amino acid solution is around its pK's?         Name four factors (bonds or other forces) that contribute to stabilizing the native structure of a protein, and describe one condition or reagent that interferes with each type of stabilizing force.     Why is silk fibroin so strong, but at the same time so soft and flexible?