You have isolated the enzyme sialidase from cell lysosomal extracts. This enzyme's activity is at its highest at pH 4.7. This pH is known as the enzyme's pH optimum. You need to study the activity of the protein in the laboratory a) Which buffer system would be the best buffer for you to use to assure the protein is the most active and why? Lactic Acid (CH3CH(OH)COOH and its conjugate base) pka 3.86 Carbonic acid (H₂CO3 and its conjugate base) pka 6.35 Phosphoric acid (H3PO4 and its conjugate base) pKa 2.14 b) Draw the disassociation of the acid you choose in water (labeling the acid form and its conjugate base) and determine the ratio of each you would need for your buffer.

You have isolated the enzyme sialidase from cell lysosomal extracts. This enzyme's activity is at its highest at pH 4.7. This pH is known as the enzyme's pH optimum. You need to study the activity of the protein in the laboratory a) Which buffer system would be the best buffer for you to use to assure the protein is the most active and why? Lactic Acid (CH3CH(OH)COOH and its conjugate base) pka 3.86 Carbonic acid (H₂CO3 and its conjugate base) pka 6.35 Phosphoric acid (H3PO4 and its conjugate base) pKa 2.14 b) Draw the disassociation of the acid you choose in water (labeling the acid form and its conjugate base) and determine the ratio of each you would need for your buffer.

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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