MacConkey agar is often used to differentiate E. coli from other Gram-negative rods. The recipe for MacConkey agar is given in the table. Suppose you plated a mixed culture of  Escherichia coli,  Bacillus subtilis, and  Streptococcus pyogenes onto a MacConkey agar plate.  Which species would grow, and how would they appear? Why would the others NOT grow?

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
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Chapter1: The Human Body: An Orientation
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 MacConkey agar is often used to differentiate E. coli from other Gram-negative rods. The recipe for MacConkey agar is given in the table. Suppose you plated a mixed culture of  Escherichia coli,  Bacillus subtilis, and  Streptococcus pyogenes onto a MacConkey agar plate. 

Which species would grow, and how would they appear? Why would the others NOT grow?

Peptone
Proteose peptone
Lactose
Bile salts (inhibits growth of gram positive cells)
NaCi
Neutral red (pH indicator, red at low pH)
Crystal violet (inhibits growth of gram positive cells)
Agar
Water
17g
3 g
10 g
|1.5g
|5g
0.03 g
0.001 g
13.5 g
1 liter
Transcribed Image Text:Peptone Proteose peptone Lactose Bile salts (inhibits growth of gram positive cells) NaCi Neutral red (pH indicator, red at low pH) Crystal violet (inhibits growth of gram positive cells) Agar Water 17g 3 g 10 g |1.5g |5g 0.03 g 0.001 g 13.5 g 1 liter
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