Mary then wanted to calculate the CFU/g of the microbes to determine their amount. She used spread plating to plate tubes 3 and 4. The table below shows the counts that she obtained after incubation. Compute for the CFU/g given the data below. Be sure to show complete solutions and box your final answer. Final answers should be expressed in 3 significant figures Tube 3 215,209 Tube 4 27,21
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- An original sample of water containing 4.00 X 106 CFU/mL was diluted by 4 successive 1/10 dilutions. After incubation, 200 colonies were found growing on the plate. How many mLs from the last dilution tube were plated out?A plate has 72 colonies with a total dilution factor of 10-7, 0.1 ml was pipetted onto the plate, what was the original CFU/ml concentration of that sample?Choose the one answer that fits best. Which of the following statements regarding the proper procedure for using Micropipettes is NOT correct? O a. You cannot use a 2-20 µl micropipette to pipet 200 µl O b. To expel all the liquid from the tip, you have to press the eject button O c. To draw up solution, press and hold the plunger at the first stop before entering the solution O d. Micropipettes always require the use of a disposable plastic tip O e. While pipetting, micropipettes should always be held as straight as possible
- A sample is diluted by a factor of 10 five times. The 10^-3 dilution has 272 colonies on it. Assuming you plated the same volume on each plate, how many colonies would you expect to find on the 10^-4 plate? Include units and use OCD formula.Andrew has to prepare 30 plates (use maximum volume), 15 slants (small test tube), and 15 stabs (big test tube) of Luria Bertani Agar. a. What is the total volume of the medium needed? b. What is the amount of each component needed, given the following media composition per liter of distilled water:Based on the following data, what is the MRSA concentration (CFU/ml) in the patient's blood sample? Write your answer in the following format: Value x 10^Exp. Do not include units. 100 μl 100 μl 100 μl 2.8X10^2 A Original 100 μl 100 μl Blood Sample 100 μl B 900 μl c 900 μl D 900 μl E 900 μl F 900 μl Saline Tubes Lawn of growth 000 100 μl 200080 100 μl 100 μl | 100 μ. | 100 με 280 22 2 0 colonies colonies colonies colonies LB + Methicillin
- An original sample of water containing 4.20 X 109 CFU/mL was diluted by 4 successive 1/100 dilutions. Exactly 1 mL from the last dilution was used to prepare a pour plate. After incubation, how many colonies were present on this plate?Prepare 50 plates of Sheep's Blood Agar using 100mm petri dish. Add 10% of blood to the media. (Manufacturer's instructions: Suspend 38 g of blood agar base dehydrated powder in 1 liter of distilled or deionized water.) Pour 25 mL of prepared medium per plate. Express answer to the nearest whole number. How many grams of the dehydrated powder should be weighed? Answer: What is the volume of distilled water that should be used in the preparation? Answer: What is the volume of sheep's blood that should be added to the sterilized and cooled blood agar base? Answer:Answer the given question below. Provide the full solution. See attached photo for the given values to be computed. A chicken salad was picked from a local subway and brought to the laboratory. 200 grams were addedto 200 ml of sterile buffered peptone water and homogenized for 2 min. The solution was chilled toprevent overheating. The final dilutions and the collected data are presented below. The plate mediumis TSA agar. Answer parts (a), (b), and (c). (a) There are ______________ CFU per ml of homogenate. (b) There are_______________ CFU per gram of the chicken salad. (c) What does CFU stand for? ______________________
- With a plate count average of 297 and a final dilution on the plate of 1:1,000 ; what is the viable number of cells in original culture (CFU/ml)?A nurse reconstituted a vial of 750 mg Cefuroxime Sodium Powder for Injection with 6mL of sterile water for injection. The reconstituted solution was dark amber-colored solution. The package insert states that solution colors range from clear to yellow depending on concentration, diluent, and storage conditions. The nurse was then hesitant to give the patient the solution due to its unusual dark color. Five portions were taken from a batch of cefuroxime sodium. Prior to testing in the instrument, each part was subjected to one of the following conditions: Conditions Specifications Temperature Portion 1: 8°C ± 2°C Portion 2: 30°C ± 2°C Portion 3: 40°C ± 2°C Light Portion 4: Kept in the dark Portion 5: Exposed to direct sunlight 1. Which form of the product – the powder for injection, the reconstituted product, or both – will you subject to the following conditions? Why do you think these forms can best address the problem?The table below summarizes the results for Lead-sulfide test. Provide the correct remarks from the results if Negative for Lead-sulfide test or Positive for the Lead sulfide test and it's either Negative for -SH or Positive for -SH