Jon performed a pour plate technique to determine the concentration of viable cells in her mother culture for nata de coco production. Prior to all the samples, he poured an extra Petri dish without any sample with the molten agar. What is the purpose of doing this?

You were instructed to add 1.0ml out of 4.0ml of an undiluted sample to 99ml of sterile diligent you add the entire 4.0ml to 99 ml. a) what was your intended dilution factor? b) what was your actual dilution factor?

What are the possible reasons why culture plates may have too many colonies despite performing serial dilutions and OD600 readings?

The following is result from a standard plate count. What is the cell density of the undiluted culture? Please follow all SPC rules. (TNTC: too numerous to count)

What are the different manufacturing methods of Suppositories dosage form? Explain heat molding process using flow chart.  Please explain at your own words.

1- Why was the T4 and E.coli mixed together in top agar and then poured on the plate instead of just spreading the two together with a glass spreader on the plate? 2- When doing the titration and isolation of the T4 phage, E.coli diluted for you to 10e7 cells/ml. why was a diluted culture used? 3- After doing the titration of T4 phage, you find your dilution plates are all TNTC. how could this have happened? 4- Could you isolate phages that infect E.coli from raw sewerage? explain.

A culture with approximately 4x105 cells/mL were incubated. After 10 hours, the number of cells had increased to 5x109. a) How long was the generation time in minutes?b) How many generations have occurred?

When testing the efficacy of an antibiotic against bacteria on an agar plate, it is important to spread the bacteria evenly across the plate before you add the antibiotic. Why do the bacteria need to be applied evenly?     None of the following are true     All of the following are true     If you add more bacteria in some areas compared to others, it might obscure the effect of the antibiotic     If the area right next to the antibiotic initially received relatively few bacterial cells, that might lead you to overestimate the effectiveness of the antibiotic     If the area right next to the antibiotic initially received a relatively large number of bacterial cells, that might lead you to underestimate the effectiveness of the antibiotic

In order to do electron microscopy the samples had to be specially prepared. Were the cells alive at the time of viewing? Explain why you said yes or no I need help answering this queshtion the answer is with the article and it has to be as short as possible  URL: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC106848/

In this experiment, different concentrations of sucrose are first prepared. Then, cylinders of potato (Solanum tuberosum) tuber are made using a cork borer, which are then trimmed to remove the skin and be of the same length. The cylinders are blot dried and weighed prior to being placed in each of the test tubes containing the sucrose solution. After 20 minutes, the cylinders were removed from the solution, blot dried, then weighed again. Study the results of the experiment shown in Table 1. Table 1. Changes in the weights of potato tuber tissue immersed in various concentrations of sucrose for 20 minutes Concentration (M) 0.0 0.2 0.4 0.6 0.8 1.0 Initial weight 3.76 3.15 3.29 3.82 3.40 3.52 Final weight 4.44 3.40 3.09 3.32 2.71 2.57 Change in weight (g) 0.68 0.25 -0.20 -0.50 -0.69 -0.95 % Change in weight 18.09 7.94 -6.08 -13.09 -20.29 -26.99 Calculate the water potential of the potato tuber cells. Express the answer in both bars and megapascal