Is the phenylethylalcohol agar (PEA) a complex or defined medium? Please help explain based on  what kind of microorganisms it allows to grow.

Hypothetically make the Dichotomous key for Micrococcus luteus, Bacillus subtilis, Bacillus, Megaterium, Bacillus cereus, E.coli, Serratia Marcescens, and Enterobacter aerogenes based on colony morphology. Starting from the results of gram staining, catalase tests, (lactose, sucrose and glucose fermentation tests) and other biochemical tests for their accurate identification.

What are Halophilic Bacteria? How can we isolate Halophiles bacteria using selective media? Please answer at your own easy words. Answer should be to the point( maximum 100-150 words)..

What is the difference between selective and differential media? Give one example of each.

With regard to studying the mechanism of conjugation,what is the purpose of using a U-tube?

Use the following passage to answer 2 consecutive questions. A urine specimen was collected in a container and placed on the central desk for delivery at 10:00 am. The specimen is not sent to the lab until 5 hours later (3:00 pm). The sample time was marked by the central clerk as collected at 4:00 pm. The bacterial species has a generation time of 30 minutes at room temperature. There are 100 bacterial cells/ml at the time of collection (10:00 am). The laboratory will cite a bacterial infection at more than 1000 bacterial cells/ml.

Describe characteristics of Proteus Vulgaris in the Agar: (How does colonies look like (color) and explain does it grow on that agar.  Blood Agar (Aerobic) MacConkey EMB PEA Mannitol Salt Agar Chocolate Agar Nutrient Agar

You have a starter culture containing 8 x 109 cells per mL, from which you take 10mL to inoculate a fresh 1 L culture. After 15 hours, the cell density of the new culture is 3 x 1012cells per mL. What are the generation time and the mean growth rate constant of theorganism in culture?

Which bacterial culture (Lb. plantarum, P. acidilactici, or P. pentosaceus) was most effective against monocytogenes and Salmonella? Explain why. What are the limitations of using an agar disk diffusion assay to assess the effectiveness of an antiseptic, disinfectant, or, in this case, a biological control agent on the growth of bacteria of interest?

Which culturing method provided the clearest results, the semisolid medium tube or the soft agar plate? Explain.