In the lactase simulation you conducted in Part 1 of this Nutrition Lab, you found that enzyme activity is dependent upon the temperature of the environment. For Part 2 (today's experiment), what would have happened if you had refrigerated your samples, keeping them at a temperature of 4°C (39.2°F) instead of at room temperature?

How can we tell if the enzyme has been denatured by a particular temperature treatment.

Use the graph below to determine which of the following is the optimal temperature for catalase. Catalase Activity 1.5 1.4 1.3 1.2 1.1 1.0 0.9 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 10 20 30 40 50 60 Temperature (°C) O 0.8 O 1.45 O 37 O 20 Percent Increase in Oxygen per Minute

9) The diagram below represents events involved as energy is ultimately released from food. Food molecule within digestive system xxx X-XX cool wat Food molecule outside of body x-xx XX-X A) 1 B) 2 The enzyme amylase will affect the breakdown of carbohydrates, but it will not affect the break- down of proteins. The ability of an enzyme molecule to interact with specific molecules is most directly determined by the (1) shapes of the molecules involved (2) number of molecules involved (3) sequence of bases present in ATP (4) amount of glucose present in the cell 6 A^XXXX X Which row in the table below best represents the chain of Xs and letters A and Bin the diagram? X-X-X-X-X-X-X A and B (1) nutrient antibodies (2) nutrient enzymes (3) hemoglobin wastes (4) hemoglobin hormones C) 3 In the human body, oxygen is absorbed by the lungs and nutrients are absorbed by the small intestine. In a single-celled organism, this absorption directly involves the (1) nucleus (2) chloroplasts Portion of…

Please handraw this graph with all the necessary detailed information:   Imagine that I text enzyme rate for four different temperatures: 10 degrees celsius, 20 degrees celsisus, 30 degree celsius, and 40 degree celsius, in separate tubes. The enzyme appears to work faster as temperature increases, but completely ceases activity at 40 degrees celcius. Sketch a graph to show this outcome, but here you will graph product formation (nmoles/mL) vs. time (minutes). The graph should be 4 lines and HANDDRAWN. Include a legend if necessary. You do not need precise quantitivate values, but most show the correct trends on the graph.

The 2 eynzymes are affected by a substrate. Choose all correct answers.

An E. coli cell with a non-functional FtsH protease would be expected to: Be unable to initiate a heat-shock response O Be unable to turn off the heat shock response once started Be able to mount a fully functional heat shock response Be unable to fix misfolded proteins during the heat shock response

Look for a real-life application of enzyme action in your daily lives. Briefly describe what happens in each

For an experiment where you use different concentrations of catalase to determine how that affects rate of the reaction with its substrate, hydrogen peroxide, what effect does enzyme concentration have on reaction activity? Would it be possible to have too much catalase in the tube? What would happen?

J. C. Servaites, in Plant Physiol. (1985) 78:839–843, observed that Rubisco from tobacco leaves collected before dawn had a much lower specific activity than the enzyme collected at noon. This difference persisted despite extensive dialysis, gel filtration, or heat treatment. However, precipitation of the predawn enzyme by 50% (NH4)2SO4 restored the specific activity to the level of the noon-collected enzyme. Suggest an explanation.