For an experiment where you use different concentrations of catalase to determine how that affects rate of the reaction with its substrate, hydrogen peroxide, what effect does enzyme concentration have on reaction activity? Would it be possible to have too much catalase in the tube? What would happen?
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- O Ara (Alt+ Ğ) Yeni. - Gözden Geçir Görünüm Yardım Başvurular Posta Gönderileri 1) Discuss this graph in the meaning of substrate depletion. 100 80 [S] [P] 60 40 20 20 40 60 80 100 Time (s.) ere goz atın 7SUS 17 19 5 8. 9. Relative Concentration20191019 1. What is the component in the SIM deep tubes that makes this medium suitable to detect the production of indole by bacteria?after a 5 g onion sample is divided into two equal parts, heat treatment to one of these partsit is being implemented. Then both heat-treated and non-treated parts are 10 times their weightit is ground in an environment containing as much buffer and the determination of pyruvate in the supernatants obtainedit's being done.a) As a result of measurements taken at 520 nm; absorbance value for the heat-treated sample;it was found that the absorbance value in the sample that was not heat treated was 0.123 and the absorbance value in the sample that was not heat treated was 0.520.The equation of the calibration accuracy for pyruvate is that y = 0.1367x – 0.001 (nmol/mL)according to the onion sample; a) independent of alinase activity; b) dependent on alinase activityand c) calculate the total pyruvate amounts in terms of nmol/g of onion.b) How is there a relationship between the activity of the enzyme allinase in onions and the amount of pyruvate?Dec,please explain.
- Rx Of oxacinophtalmic solution 3% Disp. 10 mL How many milligrams of axacin contained in each milliter of the dispensed prescription?A client reqquires uround cleansing hydrugen peroxide Bio) . with strength In order to prepare enough solutiun to do two wond treatments, you will prepar e 500mL of 3olwtim Normal saline. using In this sıtuation, what is the solute ? Solvent ? solutiun ? )escribe the method fur miscing 4his Soluutim, neluding your calculatiuns.It is required to recover active extracellular enzymes from a completed fermentation mediumthat also consists of proteins (<200,000 molecular weight cut-off (MWCO)) and bacterial cells (1-2μm). A cross-flow 0.35 μm microfiltration (MF) with retentate recycled back to the fermenter willbe used over a period of 5 hours during which time flux is assumed to decline linearly. After 5 hours, assuming 95% of the bacteria are retained and given the additionalinformation below, what is the concertation of bacteria remaining in the fermenter and what area,in m2 , of MF membrane is needed? Initial bacterial concentration = 1.2 gdcw /L (dcw = dry cell weight)Volume of medium at the beginning = 15 m3Volume of medium left in the fermenter after 5 hours filtering = 1 m3Initial (starting flux) = 6.5 x 10-5 m sec -1Final flux (after 5 hours of filtering) = 3.9 x 10-5 m sec -
- 5.15. The following data were obtained when glucose (C6H₁2O6) was added to a batch culture of microorganisms. Determine the reaction order for the disappearance of glucose. TIME. min 0 10 20 30 40 50 CONCENTRATION, g/m³ Glucose Cells 100 67 50 40 33 29 1500 1516 1525 1530 1534 1535PTP1B Substrate kcat Km kcat/Km UM 10-7 x (s-1 M) DADEPYLIPQQG DADAPYLIPQQG DAAEPYLIPQQG AAAAPYLIPQQG 44.6 + 1.8 39.8 + 0.32 3.9 + 0.9 13.7 + 0.46 1.1 + 0.25 0.29 + 0.01 35.3 + 0.22 6.6 ± 0.22 0.53 + 0.02 34.7 + 0.25 52.7 ± 0.7 0.066 + 0.001 (d) (. ) The units for kcat/KM in the above are given according to standard scientific notation. On this basis what is the value of this kinetic parameter for the DADEPYLIPQQG substrate?Calculate the Km of the enzyme with these parameters. kcat = 130s^-1 Vo = 3.0 μMs-1 S = 10 μM Et = 0.09 µM
- 2 NDC 0186-0743-31 Keep containigdy closed. PRILOSec Protect from light and moisture Store between 15°C and 30°C (599 and 80°F) OMEPRAZOLE) 30 Delayed-Release Capsules 40 mg Rx only Manufactured for AstraZeneca LP PROSE Wington, DE 10850 40 By MERCK & CO. Whitehouse Shanon, NJ 06809, USA Name AstraZeneca 11 0.4 ESCOFF Figure 6-A2 Drug label for Prilosec 1) Read the drug label in Figure 6-A2. How many Prilosec (omeprazole) capsules would contain 0.32 g of the drug? 2) Using a formula estimate the body surface area of a person whose height is 6 feet 3 inches. and who weighs 200 pounds. |||| 0.5 USUAL ADULT DOSAGE: See package insert NDC 0054-3542-58 The PROSEC (omeprazole) Delayed Release Capsule should be swallowed whole and not opened, chewed, or crushed. 4) Order: Oncovin (vincristine sulfate) 2.5 mg po daily. The BSA is 1.63 m2 and the recommended dose is 1.4 - 2.0 mg/ m2. Is the prescribed dose in the safe dose range? 5) Indicate with an arrow a reading of 0.42 mL on the scale…Aerobic degradation of an organic compound by mixed cultureof organism in wastewater can be represented by following reaction. C3H6O3 + a O2 + b NH3 → c C5H7NO2 + d H2o + e CO2 A. Determine a, b, c, d and e, if YX/S = 0.4 d X/g S. B. Determine the yield coefficients YX/O2 and YX/NH3. C. Determine the degree of reductions for the substrate, bacteria and RQ for the organismsComposition of Cell Lysis Buffer Required Concentration of Reagent Molecular Weight Volume or Weight of Reagents Needed 40 mM Tris HCl 157.59 20 mM Acetic acid 60.05 1 mM EDTA 292.24 10 mM MgCl2 from 25mM stock 95.21 5% SDS 288.37 ddH2O 18.02