In normal mitochondria, which of the following is true for the rate of NADH consumption (oxidation)? Select all that apply Be very low if the ATP synthase is inhibited, but increase when an uncoupler (equalizer of H+ concentration) is added decrease if mitochondrial ADP is depleted Increase in active muscle and decrease in inactive muscle decrease when cyanide is added to prevent electron transfer through the electron transport chain
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- The Relative Efficiency of ATP Synthesis in Noncyclic versus Cyclic Photophosphorylation If noncyclic photosynthetic electron transport leads to the translocation of 7 H+/2e- and cyclic photosynthetic electron transport leads to the translocation of 2 H+/e-, what is the relative photosynthetic efficiency of ATP synthesis (expressed as the number of photons absorbed per ATP synthesized) for noncyclic versus cyclic photophosphorylation? (Assume that the CF1CF0-ATP synthase yields 3 ATP/14 H+.)Which of the following statements about oxidative phosphorylation is correct? O H+ ions are transferred from Complex I or Complex II to ATP synthase where ATP production occ O Proton pumps transfer electrons from the cytosol to the mitochondrial matrix as electrons are tra O The chemical and electrical gradient is established between the intermembrane space and the ma electron carriers. O ATP synthase pumps electrons back to the intermembrane space as a consequence of electrocher mitochondrial matrix. • PreviousIn the presence of excess oxygen, a complete oxidation of seven molecules of glucose into carbon dioxide and water, by a yeast cell, would produce approximately ATP molecules via oxidative phosphorylation only. (Consider NADH = 3 ATP and FADH2 = 2 ATP)
- When rotenone is added to actively respiring mitochondria,the ratio of NADH to NAD+ increases, but the FADH-/FADratio remains unchanged. What step in the system is beinginhibited?C000 C000 NADH NAD -NADH NAD* oco, NAD Oco,- FADH NADH FAD ATP ADP+,P Select the conclusion that would most accurately explain the impact on the Krebs Cycle if glycolysi were to slow down its rate of reaction. ATP production would increase since glycolysis would be using less ATP. The Krebs cycle production of FADH2 would increase. O The Krebs cycle would continue to function at a normal rate. O The Krebs cycle production of NADH would decrease.If K* and valinomycin are added to respiring cells, fully coupled ATP-synthesizing mitochondria, explain what will happen to the pH gradient and the AY. Compare the action of valinomycin with gramicidin in ATP production and electron transport.
- Concerning the process of the aerobic fate of glucose oxidation, which of the following statements is false? O The spontaneous movement of protons is coupled to the unfavorable movement of electrons through the electron transport chain O The matrix of the mitochondria is more negative than the inner membrane space O Pyruvate is recognized bya pyruvate/proton symporter for movement into the matrix of the mitochondria O The movement of protons through ATP synthase drive the production of ATP O Mitochondria contain two membranes, only the inner mitochondrial membrane is highly selective for what passes across itA poison that prevents the transfer of electrons from the last [Fe-S] cluster of Complex I to coenzyme Q is added to a suspension of actively respiring mitochondria. Which of the following will be observed? ATP production would be impaired due to the uncoupling of oxidative phosphorylation from electron transport. ATP production would be reduced due to inhibition of the CoQ subunit of ATP synthase. ATP production would be reduced due to a decrease in the number of protons pumped out of the mitochondrial matrix. ATP production would be halted completely due to the block in electron transport through the electron transport chain.Concerning the process of the aerobic fate of glucose oxidation, which of the following statements is false? The spontaneous movement of protons is coupled to the unfavorable movement of electrons through the electron transport chain O The matrix of the mitochondria is more negative than the inner membrane space Pyruvate is recognized by a pyruvate/proton symporter for movement into the matrix of the mitochondria O The movement of protons through ATP synthase drive the production of ATP O Mitochondria contain two membranes, only the inner mitochondrial membrane is highly selective for what passes across it
- Give all the reactions that will produce ATP either by substrate-level phosphorylation (SLP) or by oxidative phosphorylation (OP). If the given require a shuttle system, please indicate both MA shuttle and GP shuttle and give the ATP produced. Given: glucose 6-phosphate to 2succinly CoAIf decanoic acid (10 : 0) undergoes complete β-oxidation in mitochondria, how many net ATP is yielded? (NADH = 2.5 ATP; FADH2 = 1.5 ATP; Acetyl CoA = 10 ATP) Answer in number onlyIf there is ample oxygen available, how many ATP can be produced from complete oxidation of glucose (from glycolysis up to ETS)? Assumption: 1 NADH = 3 ATP; 1 FADH2 = 2 ATP