Homework Help is Here – Start Your Trial Now!
Science
Biochemistry
Recombinant protein is produced by a genetically engineered strain of Escherichia coli during cell growth. The recombinant protein can be considered a product of cell culture even though it is not secreted from the cells; it is synthesised in addition to normal E. coli biomass. Ammonia is used as the nitrogen source for aerobic respiration of glucose. The recombinant protein has an overall formula of CH1.55O0.31NO.25. The yield of biomass (excluding recombinant protein) from glucose is measured as 0.48 g g21; the yield of recombinant protein from glucose is about 20% of that for cells. а. How much ammonia is required?

Recombinant protein is produced by a genetically engineered strain of Escherichia coli during cell growth. The recombinant protein can be considered a product of cell culture even though it is not secreted from the cells; it is synthesised in addition to normal E. coli biomass. Ammonia is used as the nitrogen source for aerobic respiration of glucose. The recombinant protein has an overall formula of CH1.55O0.31NO.25. The yield of biomass (excluding recombinant protein) from glucose is measured as 0.48 g g21; the yield of recombinant protein from glucose is about 20% of that for cells. а. How much ammonia is required?

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
See similar textbooks
icon
Related questions
Question

only number 2

b My Questions | bart X * Google Translate O SSO - Dashboard Ti General ([SIAP-202 X 6 (6) WhatsApp A Dasar-dasar Biopro X + A drive.google.com/drive/folders/10_DzC9YxnUNJnuvolJj-xOawSTUSkITz stoichiometry: a) Determine the concentration and total amount of glucose and (NH4)2SO4 in the nutrient medium. b) Determine the yield coefficients Yx/s (biomass/glucose) and Yxo2 (biomass/oxygen). c) Determine the total amount of oxygen required. d) If the rate of growth at exponential phase is rx= 0.7 gdw/l-h, determine the rate of oxygen consumption (g O2/l-h). -> Recombinant protein is produced by a genetically engineered strain of Escherichia coli during cell growth. The recombinant protein can be considered a product of cell culture even though it is not secreted from the cells; it is synthesised in addition to normal E. coli biomass. Ammonia is used as the nitrogen source for aerobic respiration of glucose. The recombinant protein has an overall formula of CH1.55O0.31No.25. The yield of biomass (excluding recombinant protein) from glucose is measured as 0.48 g g21; the yield of recombinant protein from glucose is about 20% of that for cells. 2. a. How much ammonia is required? b. What is the oxygen demand? If the biomass yield remains at 0.48 g g21, how much different are the ammonia and oxygen requirements for wild-type E. coli that is unable to synthesise recombinant protein? с. 3. The following data are obtained in oxidation of pesticides present in wastewater by a mixed culture of 5 1 10 Q + microorganisms in a continuously operatng aeration tank Page 26°C 19:56 LINE Berawan 25/04/2022 .. >
Transcribed Image Text:b My Questions | bart X * Google Translate O SSO - Dashboard Ti General ([SIAP-202 X 6 (6) WhatsApp A Dasar-dasar Biopro X + A drive.google.com/drive/folders/10_DzC9YxnUNJnuvolJj-xOawSTUSkITz stoichiometry: a) Determine the concentration and total amount of glucose and (NH4)2SO4 in the nutrient medium. b) Determine the yield coefficients Yx/s (biomass/glucose) and Yxo2 (biomass/oxygen). c) Determine the total amount of oxygen required. d) If the rate of growth at exponential phase is rx= 0.7 gdw/l-h, determine the rate of oxygen consumption (g O2/l-h). -> Recombinant protein is produced by a genetically engineered strain of Escherichia coli during cell growth. The recombinant protein can be considered a product of cell culture even though it is not secreted from the cells; it is synthesised in addition to normal E. coli biomass. Ammonia is used as the nitrogen source for aerobic respiration of glucose. The recombinant protein has an overall formula of CH1.55O0.31No.25. The yield of biomass (excluding recombinant protein) from glucose is measured as 0.48 g g21; the yield of recombinant protein from glucose is about 20% of that for cells. 2. a. How much ammonia is required? b. What is the oxygen demand? If the biomass yield remains at 0.48 g g21, how much different are the ammonia and oxygen requirements for wild-type E. coli that is unable to synthesise recombinant protein? с. 3. The following data are obtained in oxidation of pesticides present in wastewater by a mixed culture of 5 1 10 Q + microorganisms in a continuously operatng aeration tank Page 26°C 19:56 LINE Berawan 25/04/2022 .. >
Expert Solution
steps

Step by step

Solved in 2 steps

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Recommended textbooks for you
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON
SEE MORE TEXTBOOKS