In a mixed copolymer experiment, messages were created witheither 4/5C : 1/5A or 4/5A : 1/5C. These messages yielded proteinswith the following amino acid compositions.4/5C : 1/5A (% yield) 4/5A : 1/5C (% yield)Proline 63.0 3.5Histidine 13.0 3.0Threonine 16.0 16.6Glutamine 3.0 13.0Asparagine 3.0 13.0Lysine 0.5 50.098.5 99.1Using these data, predict the most specific coding composition foreach amino acid.

The pathway for arginine biosynthesis in Neurospora crassa involves several enzymes that produce a series of intermediates as shown. O O O O ornithine citrulline ARG-E arginosuccinate arginine N-acetylornithine arginine You did a cross between ARG-E ARG-H* and ARG-E* ARG-H¯¯ Neurospora strains and identified an Arg- strain from an NPD tetrad. (Assume that Neurospora forms tetrads in the same way yeast do.) Which compound would rescue growth of this Arg- spore? N-aceltylornithine ARG-F ornithine citrulline ARG-G ARG-H → argininosuccinate

Droplet based single cell DNA sequencing uses a microfluidic platform which generates droplets encapsulating individual cells. A micrograph of such a device [Zilionis et al, Nature Protocols 2016] is presented in Figure Q3. The channels are 25 μm deep. (a) (b) Oil Figure Q3. Micrograph of the microfluidic droplet device. Scale bars are 100 µm. Adapted from Zilionis et al, Nature Protocols 2016. Red arrowheads show individual cells, and black arrows indicate flow direction. (c) RT/lysis reagents Cells Barcoding hydrogel beads A company wants to explore the design of a prototype of the device made from PDMS. Describe a suitable fabrication process, explaining the rationale behind each individual step. Schematics can be drawn to illustrate your response. You may want to refer to the datasheets at the end of the script. Testing of the prototype was successful and the company wants to explore the feasibility of manufacturing the device from glass. Describe the fabrication process for this…

We isolate DNA from a bacteria that is wild type for the genes arg leu and his shear it in a blender mix it into a culture of bacteria that is mutant for the genes arg leu and his . We then plate on various media. These are the results: Media with arginine 20 colonies /ml Media with histidine 300 colonies /ml Media with leucine 500 colonies /ml Media with all three 10000 colonies/ml 21. What is this experiment called? A. transpiration B. conjugation C. transformation D. transduction