Immunostaining was used to investigate the expression of the Fibrillin 1 gene in muscle fibres. Twoantibodies were used, Antibody A binds to a muscle membrane protein that is unrelated to Fibrillin.Antibody B binds to the N terminus of Fibrillin 1, whilst Antibody C binds to the C terminus of Fibrillin 1.What is the best explanation for the staining pattern see in the following figureAntibody BAntibody AAntibody C A point mutation in the fibrillin gene introduced a stop codon in the proteinA gene translocation has removed the N terminus of the proteinA gene translocation has removed the chromosome encoding the fibrillin geneA tuncation mutation has removed the central region of the proteinThe C and N termini of the Fibrillin protein are unchanged by the mutationA point mutation in the fibrillin gene has changed an amino acid in the proteinA point mutation in the fibrillin gene has introduced a start codon in the proteinA truncation mutation has removed the N terminus of the proteinA point mutation in the fibrillin gene has resulted in the removal of a stop codonBoth the N and C termini regions of the protein have been deleted by a mutationA point mutation in the fibillingene has led to it not being expressed

Immunostaining is a process of staining proteins by antibodies that are covalently linked to an…

Answered: Immunostaining was used to investigate…

Curren'S Math For Meds: Dosages & Sol

11th Edition

ISBN:9781305143531

Author:CURREN

Publisher:CURREN

Chapter16: Adult And Pediatric Dosages Based On Body Surface Area

Section: Chapter Questions

Problem 7.2P

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You have the following reagents available Rat anti-Keratin 8 antibody specific for mouse Keratin 8, Rabbit polyclonal antibody against mouse keratin 5 and antifade reagent with DAPI to stain DNA. Both primary antibodies work best at 1:500 dilution. A) What additional reagents would you need to perform immunostaining of frozen sections of mouse thymus tissue for Keratin 8 that is expressed in cortical thymic epithelial cells and Keratin 5 that is expressed in mouse medullary thymic epithelial cells? B) Create a staining protocol including all tubes and appropriate controls to stain 4 slides with frozen thymic sections. You will need 200ul of antibody staining solution for each slide.
When a mixture of different IgG antibody proteins are treated with the enzyme papain, each antibody is cleaved into three roughly equal size fragments. From each original antibody, two of the three fragments are identical to each other, and represent the ‘arms’ of the antibody ‘Y’. These fragments are known as Fab fragments. The third fragment is known as the Fc region, because this fragment will crystallize when purified. The reason a mixture of Fc fragments will crystallize is because: It is the only part of the antibody protein that can easily be purified at the high concentrations needed for crystallization. It has no disulfide bonds holding the domains together, as disulfide bonds will inhibit crystallization. It is the only fragment of the antibody that still has disulfide bonds, so it remains intact during the crystallization process. The Fc fragments of IgG are much more water soluble than the Fab fragments. All Fc fragments generated from a mixture of IgG molecules have the…
Under the microscope, the fluorescent signal from an isolated quantum point will form a stain: A.Approximately the same size as a fluorescent protein of the same colourB.Much smaller than a fluorescent protein, regardless of colourC.Relatively dark compared to antibodyD.Which corresponds approximately to the size of the quantum point, 20 to 40nm.
Have a look at the results in this SDS-PAGE gel. A MW ladder is shown. In the "control" lane, a sample of recombinant protein "LBT3" was loaded. Lane A shows proteins that have been prepared from leukocytes from a normal, healthy individual. Lanes B, C, D, E and F show protein prepared from leukocytes from individuals with lymphoma. What is the best explanation for the result shown for patient D? 200 150 100 75 50 37 20 Weight, kDa MW ladder Control A
After the electrophoretic separation, the proteins are transferred to a nitrocellulose membrane prior to staining with antibodies specific for the protein understudy. Describe in detail how the primary and secondary antibodies could aid in the protein detection process.
An example of dosage as it applies to blood group antigens is: Question 2 options: Every antibody specificity will react with cells with a homozygous expression Some antibody specificities may only react with an antigen with homozygous expression Some antibody specificities may only react with an antigen with homozygous expression Every antibody specificity will react with cells with a heterozygous expression
In the more common protocol for immunofluorescence detection of cellular proteins, an investigator uses two antibodies. The first binds specifically to the protein of interest. The second is labeled with fluorochromes for easy visualization, and it binds to the first antibody. In principle, one could simply label the first antibody and skip one step. Why use two successive antibodies?
What phi and psi angles would you predict to be most prevalent for myoglobin and green fluorescent protein, in a Ramachandran plot
What internal references can be used in western blot of plasma membrane protein,expect Na-K ATPase??
A unique aquatic plant was discovered from a lagoon in El Nido, Palawan. To determine the protein content of the plant, an adequate amount of the plant extract was acquired, and Bradford assay was performed. Determine the total protein concentration, in µg/mL, of the acquired plant extract. A bovine serum albumin (BSA) stock solution with a concentration of 250 µg/mL was used and mixtures with the following compositions and absorbance readings were prepared: Volume of BSA (mL) Volume of water (mL) Absorbance Tube # at 595 nm 1 0.00 2.00 0.000 2 0.20 1.80 0.112 3 0.40 1.60 0.225 4 0.60 1.40 0.318 5 0.80 1.20 0.432 6 1.00 1.00 0.551 The absorbance reading of the plant extract is 0.275.
Why might it be necessary to include the 50 mL cultures in order to express protein? This question is referring to a lab experiment for protein expression. We streaked our plates. Put 50uL of two antibodies (AMP and CAM) into 50mL LB broth. Then took a colony and mixed it in the flask with LB broth and antibodies. The next step is to incubate the flasks overnight. Then add 500uL of AMP to 500mL of LB broth. We will add 25mL of the overnight culture to the 500mL LB broth. Hopefully this is enough detail to answer the question. Thank you!..
This is an SDS-PAGE of an antibody purification sample with IgG seperated from a Bovine Calf serum. Would you be able to describe the bands that are appearing and why it appeared on the gel this way? As well, a western blot was done after the SDS-PAGE and the bands that appeared were at 50 kDa and 150 kDa for the IgG and two elution lanes. May you please explain?

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