Have a look at the results in this SDS-PAGE gel. A MW ladder is shown. In the "control" lane, a sample of recombinant protein "LBT3" was loaded. Lane A shows proteins that have been prepared from leukocytes from a normal, healthy individual. Lanes B, C, D, E and F show protein prepared from leukocytes from individuals with lymphoma. What is the best explanation for the result shown for patient D? 200 150 100 75 50 37 20 Weight, kDa MW ladder Control A

Have a look at the results in this SDS-PAGE gel. A MW ladder is shown. In the "control" lane, a sample of recombinant protein "LBT3" was loaded. Lane A shows proteins that have been prepared from leukocytes from a normal, healthy individual. Lanes B, C, D, E and F show protein prepared from leukocytes from individuals with lymphoma. What is the best explanation for the result shown for patient D? 200 150 100 75 50 37 20 Weight, kDa MW ladder Control A

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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If a student uses a monoclonal antibody to detect a protein using a direct Western-blotting protocol after running an SDS PAGE, the student can detect a 50kd protein. However, if the student tries to use this antibody to immunoprecipitate the 50 kd protein and then Western -blot the immunoprecipitated samples, the student found that there is no band at all at 50kd region in the Western blot. How do you explain these results based on what you know about protein structure and antigen-antibody recognition? (Hint: Western blot analysis explores an SDS-containing buffer, whereas the immunoprecipitation buffer uses a NP40-containing buffer to maintain proteins in their native form).
True of False 9) Gels are read from left to right. Answer: 10) Functional segments of genes (introns) code for proteins, molecules that carry out most cellular functions. Answer: 11) The human genome contains small, repetitive DNA elements that have become randomly inserted into it over millions of years. Answer:
The schematic on the right is for which molecular biology method? What information does this method reveal?
In size exclusion chromatography, which component will elute last in the set up? O Small proteins will elute last because they do not pass through the pores of the gel beads. O Large proteins will elute last because they pass through the pores of the gel beads. O Small proteins will elute last because they pass through the pores of the gel beads. Large proteins will elute last because they do not pass through the pores of the gel beads.
Please explain how different detergents take out proteins selectively out of membrane. (Choose one protein as an example from the figure)
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B1
Please paraphrase how both experiments (from fig.2 and 3) are asking differing questions