If you wanted to genetically engineer higher metabolic production of a target end product 'X', how would you do this?how would your approach differ in prokaryotes versus eukaryotes ?
Q: What is an exconjugant? How do you think that exconjugants were obtained? (It might include genes…
A: Answer: Introduction: Conjugation is the process of transfer of DNA from one bacterium to other by…
Q: Suppose that one species of bacteria obtained one gene encoding a restriction endonuclease by…
A: The origin of new genes in an organism is essential for their evolution. These new genes are…
Q: An archaeon (Sulfolobus acidocaldarius) found in acidic hot springs contains a topoisomerase that…
A: Topoisomerases are the enzymes that participates in the underwinding or overwinding of DNA. It…
Q: What is the role of the origin of transfer during F+- and Hfr-mediated conjugation? What is the…
A: Conjugation is a process of transfer of genetic material between two bacteria. This transfer can…
Q: Suppose that E. coli sustains a mutation in its gene for the lac operon repressor such that the…
A: An operon is the unit of bacterial gene expression and regulation. The operon contains the…
Q: What will happen if the parts of ubiquitin-conjugating enzymes' parts change specifically the (…
A: Ubiquitin is a 76 amino acid polypeptide that is found in a eukaryotic cell. ubiquitin is served…
Q: .Let's compare the proteome of Chlamydomonas with those of humans and the plant Arabidopsis. Which…
A: Chlamydomonas is a genus of single-celled green alga found in soil, ponds, ditches, and even in…
Q: A group of genetics students mix two auxotrophic strains of bacteria: one is leu+ trp+ his− met− and…
A: Bacteria are a group of prokaryotic microscopic single celled organisms. They live in diverse…
Q: Scientists are using metagenomics to tackle one of themost significant problems affecting human…
A: Metagenomics is a field of science that deals with studying genetic material present in samples…
Q: What role do genes, RNA, and protein synthesis play in molecular microbiology?
A: In the molecular biology the DNA, RNA and proteins are very important that determine the structure…
Q: Given the following genotypes, explain, by answering the questions in each number, how the mutation…
A: i + p + o + z - y + Complete set of gene products will not be there because z gene that codes for…
Q: How could transposons be used in the study of bacterial genetics
A: Transposition is a method of translocation of small DNA segment from one location called donor site…
Q: Where is the key difference between the reactions that result in the final product of fermentation…
A: Biomolecules are the biological molecules that are present inside the living organisms. These…
Q: What is abiogenesis? Why the Urey-Miller experiment so important? How did the experiment work?…
A: Introduction :- According to the theory of abiogenesis, the earliest life forms produced were…
Q: Why would it be an advantage to synthesize eukaryotic genes byusing reverse transcriptases to make…
A: Reverse transcriptase is a RNA based DNA polymerase used to convert RNA into cDNA and this process…
Q: Which of the following prokaryotic cells would probably not be “competent” at transformation? an…
A: Bacteria can take up foreign DNA in a process called transformation. Transformation is a key step in…
Q: Imagine that there is an E. coli outbreak in your area, and you would like to test the kangkong from…
A: E coli or Escherichia coli is basically a bacterium commonly found in lower intestine of warm…
Q: In your laboratory, you have an F− strain of E. coli that is resistantto streptomycin and is unable…
A: Bacteria are prokaryotic organisms that have undefined nucleus and nuclear membrane. Most of the…
Q: Why is genetic transfer important for the survival of prokaryote species?
A: The prokaryotic organisms are mainly the bacteria that do not contain any membrane bound cell…
Q: Name three ways in which genetic information of bacteria can be changed.
A: The change in genetic information in bacteria is called transformation. It may occur by three…
Q: Based on the images attached, what does the similarity in the sequences tell you about the ermB gene…
A: A cladogram is a branching tree diagram that depicts a phylogeny estimation of the evolutionary…
Q: Name 3 ways in which genetic information of bacteria can be changed?
A: The method of genetic recombination in bacteria by which bacteria diversify their genetic material…
Q: What is bioremediation? What is the difference between biotransformationand biodegradation?
A: A microorganism is a microscopic organism, which may exist in its single-celled form or in a colony…
Q: . Genes A, B, and C encode enzymes A, B, and C, which are part of this biosynthetic pathway:…
A: If the gene A,B,C are presemt in the eukaryotes and bacteria and since 1 is prokaryotic and another…
Q: You grow E. coli in defined media containing both glucose and lactose. Draw the trajectory of the…
A: The lac operon It is a set of three genes in E.coli that code for enzymes that help the cell use…
Q: The accompanying figure illustrates the portion(s) of a typical bacterial or archaeal genome that…
A: Bacteria are microscopic organisms which belong to prokaryote because these are unicellular…
Q: Why are regulatory mutants used to increase the production of glutamic acid by Corynebacterium…
A: Corynebacterium glutamicum is a rod-shaped gram-negative bacteria of the Corynebacteriaceae family…
Q: Supercoiling of DNA requires GTP as source of energy is only observed in prokaryotes is…
A: DNA is a genetic material present in most living organisms. In case of eukaryotic cells DNA is found…
Q: You mix two auxotrophic strains of bacteria; one is leu+ trp+ his– met– and the other is leu– trp–…
A: Transduction is a process where the bacterial genes are transferred via the use of bacteriophage…
Q: Suppose that E. coli sustains a mutation in its gene for the lac operon repressor making the…
A: When lactose is not present in the medium operon does not transcribe because of the presence of lac…
Q: Suppose you want to figure out what genes are involved in the production of proline (Pro) in a…
A: Introduction :- Proline is one of the essential amino acid .It is the only proteinogenic secondary…
Q: Why do we want to genetically manipulate bacterial?
A: According to guidelines we have to answer the first question only. so please kindly post the…
Q: Why can’t a researcher use a poly-T primer in a reverse transcriptase reaction that utilizes…
A: Introduction :- Reverse transcriptase is mRNA dependant DNA polymerase enzyme. It is the type of…
Q: suggest a strategy that you might employ to isolate all of the genes involved in nitrogen fixation…
A: Nitrogen fixation is the process by which the free form of atmospheric nitrogen is converted or…
Q: Bacterial conjugation has medical consequences. For example, certain plasmids contain genes that…
A: Bacterial Conjugation refers to parasexual mode of reproduction by which the genetic material is…
Q: Bt eggplant is a genetically-modified eggplant developed by Filipino scientists. It was developed to…
A: The genetically modified eggplant is the world’s first GM eggplant to have insecticidal protein,…
Q: What are the advantages and disadvantages of transgene fish in Aquaculture?
A: A transgenic fish are those which contains genes from another species. These are the improved…
Q: Bt eggplant is a genetically-modified eggplant developed by Filipino scientists. It was developed to…
A: Bacillus thuringiensis was grown in a variety of media, including legume seeds, dried cow blood, and…
Q: Why are some eukaryotic genomes so large?
A: Eukaryotes are organisms with membrane-bound organelles in their cells.
Q: What is meant by "transformation" and how does Agrobacterium transform a plant cell? What are the…
A: "Since you have asked multiple questions, we will solve the first question for you. If you want any…
Q: An archaeon (Sulfolobus acidocaldarius) found in acidic hot springs contains a topoisomerase that…
A: DNA : It is a molecule composed of two polynucleotides chains that coil around each other to form a…
Q: Why would using dt Oligos to produce complimentary DNA (cDNA) from bacteria mean that your reaction…
A: The genetic information is stored in the DNA which undergoes semi-conservative model for…
Q: is “Agrobacterium-mediated genetic transformation” in plants described as natural genetic engineer…
A: Agrobacterium is a gram-negative bacteria that uses horizontal gene transfer to cause tumors in…
Q: Suppose that the E. coli gene for the lac operon repressor has a mutation that makes the repressor…
A: Lac operon is a concept in the E.coli bacteria which helps in transportation and metabolism of…
Q: cytosine is deaminated. Describe the outcome of this deamination and explain in detail how E. coli…
A: DNA is made up of four bases two purines and two pyrimidines. Two purines are adenine and guanine.…
Q: Why is Agrobacteriummediated genetic engineering transformation inplants considered as natural…
A: Agrobacterium is a gram-negative bacteria genus that uses the HGT (horizontal gene transfer) method…
If you wanted to genetically engineer higher
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- Imagine that there is an E. coli outbreak in your area, and you would like to test the kangkong from your local grocery store. How could you modify this protocol to extract DNA from the kangkong (to identify the species) and check for presence or absence of E. coli.? Keep in mind that (i) E. coli is free-living and not an endosymbiont, and (ii) plant cells are encased in both a cell membrane and cell wall.Bacillus subtilis is known to harbour proA gene which is expressed as protease. Protease is an industrially important enzyme but the yield from Bacillus is not satisfactory. What processes of genetic engineering can you conduct to increase the production of proA gene? Briefly discuss your vision on the context.In their Nobel Prize-winning experiments, Beadle and Tatum exposed fungal spores to X-rays to induce mutations. If the mutants had mutations in the arginine metabolic pathway, they would grow in ------minimal medium (wrong answer), but NOT in -------complete medium (wrong answer).
- What makes the species of Agrobacterium ideal for genetic engineering? Describe its characteristics and its role in producing transgenic plants.Why is Agrobacterium mediated genetic transformation described as Natural Genetic engineering in plants?Why do we want to genetically manipulate bacterial? how can we genetically manipulate bacteria? How can we tell if we have successful transaction?
- Remember that although there are many interesting ideas about genetic engineering of plants and animals, this is specifically about GE bacteria. Please be sure you are answering the following questions: 1) What is the species that was modified, and what species did the introduced genes come from? 2) What is the purpose of the genetic engineering? What were people hoping to accomplish? 3) What are the benefits (or potential benefits) of the engineered bacterium? 4) What are the risks (or potential risks) of the engineered bacterium?Suppose you want to figure out what genes are involved in the production of proline (Pro) in a certain bacteria. (1) How would you go about designing a mutant screen to do so? (2) Suppose your screen identified 5 mutants that barely grew without being given Pro in the medium. How would you go about determining the number of different genes these mutants represent?Where is the key difference between the reactions that result in the final product of fermentation to form lactic acid or alcohol? What is the significance of these differences in terms of biotechnology?
- What is an exconjugant? How do you think that exconjugants were obtained? (It might include genes not described in this problem.)Genetically modified foods are products produced from organisms that have had changes introduced into their DNA using the methods of genetic engineering. Golden rice, Flavr Savr tomatoes and Roundup Ready soyabeans are products of this technology. Discuss the advantages of these three genetically modified crops. Name ONE (1) strategy to produce transformed plant cells.Why is gene editing becoming a trend in modern genetic engineering? Provide a concrete example on your explanation. 2. Out of 4 presented methodologies in gene modification/editing, which do you think is the most promising in providing efficient result? Why do you say so? 3. What makes the species of Agrobacterium ideal for genetic engineering? Describe its characteristics and its role in producing transgenic plants. 4. In which of the following aspects do you think it is worthwhile to develop genetic engineering? Why or why not? a.) Agriculture and Food Industry b.) Medicine c.)Research d.) Entertainment 5. What are the possible bioethical issues that gene editing tools may encounter? 6. Do you think genetic engineers play God when they modify the genes of various organisms to enhance their existing traits? Why or why not?