Gram staining is the most common initial test of unknown identification, explain in your own words the process of gram staining

Q: Describe how you would perform a Gram Stain. Describe how to perform the streak for isolation…

A: According to guidelines , we are supposed to attempt first three questions in case of similar…

Q: The number of bacteria in a sample can also be quantified with other techniques, such as examination…

A: In microbiology laboratories the number of microorganisms present in a particular sample is…

Q: What method is best for for isolating a colony on a TSA plate? A. Swab plate B. Straight line…

A: An isolated colony represents a pure source of an organism from which a pure culture can be started.

Q: During a phenotypic method of identifying an unknown microbe, the specimen was noted to be positive…

A: Introduction The micro-organism is the organism that is too small to be seen with the naked eye. It…

Q: 3. Draw the specimen observed under endospore staining  4. Draw the specimen observed under…

A: Endospore staining: a. It is a differential stain used to distinguish between the vegetative cells…

Q: You have isolated a Gram positive cocci from a throat culture. You have determined that it is…

A: Gram-positive bacteria have a single cell wall anchored to the cell membrane by lipoteichoic…

Q: 3 things about each one? are they selective , differential , positive , negative results etc.…

A: Selective media will be used for growth of only selected microorganisms. Differential media are…

Q: The goal of Disc diffusion test is   Select one: to determine the suitable concentration of an…

A: Antibiotics, such as penicillins and cephalosporins, are a form of antimicrobial drugs that fight…

Q: Why do you think  rapid methods so helpful to identify pathogens microbes in clinical settings. Why…

A: Introduction: Pathogens are the microorganisms which has an ability to cause disease. Mainly…

Q: ake a look at your streak plate from the use of a mix culture. Does your plate show the results that…

A: The streak plate method is a microbiological technique used for the isolation of pure strain ;…

Q: Phase contrast microscope is linked to A: DAPI diamidino-2-phenylindole O F: All answers are wrong 0…

A: A phase-contrast method uses an optical mechanism to convert minor differences in phase into similar…

Q: Presence of this specific bacterial surface polymer is usually demonstrated as a clear area when…

A: The polymeric surface covering in bacterial cells is called as capsule, this covering is made up of…

Q: Use the differential test matrix for this question. You are performing a single unknown. You have…

A: Methyl red test is as also called as MR test. This test is used to determine if the organism…

Q: You have a culture of bacteria and you performed an Acid Fast staining. You see red rod-shaped…

A: Introduction -  Acid-fast staining is a differential stainingused to differentiate acid fast and non…

Q: What is the color result of 1% solution of casein, Albumin, Gelatin in ninhydrin test? Include…

A: Ninhydrin test: Chemical test to detect presence of ammonia, primary/secondary amines, or amino…

Q: The capsule stain is considered a negative stain

A: Staining is a technique in microbiology so as to enhance the view by contrasting it.  Stains are…

Q: one or your lab partners has followed the rrecommended procedure of running Gram-positive and Gram…

A: Gram staining is a common laboratory test to characterize the bacteria into gram-positive and…

Q: Create paragraphs to differentiate among the following terms: positive stain, negative stain, simple…

A: An organic molecule with a benzene ring, a chromophore, and an auxochrome group is known as a…

Q: PREPARATION OF SMEARS: 1. Wash the slide thoroughly with a detergent, rinse with water and dry with…

A: PREPARATION OF SMEAR ( FLOWCHART)

Q: Which of the following are true of the above picture? (Select all that apply.) V The bacteria are…

A: Gram-negative bacteria have a thin peptidoglycan layer which fails to retain colour after treatment…

Q: The acid-fast stain is used to stain _____. The decolorizer used to destain the cells is ______.…

A: The staining procedures used to differentiate the types of bacteria are known as a differential…

Q: Answer this question xi) What is the most important step in the Gram staining procedure? Why?

A: Gram staining helps in differentiating between gram positive and gram negative bacterias. The gram…

Q: A patient sample will be analyzed. You are responsible to quantify the bacteria number. The dilution…

A: A sample of your blood, urine, skin, or any part of your body will be taken during a bacteria…

Q: Types of Differential Staining techniques.  1. Gram stain 2. Acid-fast stain     Please…

A: Staining is used in microscopy to highlight the structures to be viewed under microscope. It is of…

Q: Which stain can be used to determine if bacteria are capsulated?    Question options:   flagella…

A: Many bacteria are capsulated bacteria. Capsule is one of the virulence factor present on the outer…

Q: 1.)What is the purpose of a counterstain?

A: BASIC INFORMATION  ENZYMES They are the catalyst. They help in accelerating the chemical reaction.…

Q: e the process to make a 4-phase streak plate beginning with your first streak (i.e you have…

A: The sample/inoculum is usually pored by streak with the diluted through streaking it across the…

Q: Compare and contrast gram positive and gram-negative bacteria, include physical properties,…

A: Gram staining, often known as Gram's method, is a staining method for distinguishing and classifying…

Q: step by step process of Methyl Red test using a schematic diagram

A: The Methyl Red Voges Proskauer protocol is used to identify and differentiate the microbes based on…

Q: What is the purpose of Xanthoproteic test and explain the observation or rection for albumin and…

A: Xanthoproteic test is a type of colour test to detect a specific functional group, structural…

Q: Which of the following techniques is not used to count bacteria?         Optical density…

A: Bacterial counting can be done by via- (1) Optical density- Optical density of bacteria can be used…

Q: Which of the following can be used to distinguish one colony from another on an agar plate?  Select…

A: A colony is a visible cluster of microorganisms like fungi and bacteria that grow on a solid growth…

Q: Which of the following is not meant to reduce contamination of yoru sample? avoiding hand contact…

A: A microbial culture is done to multiply the specific strains of the microorganisms under specific…

Q: in most probably number (MPN) testing what media is used for confirming test and what does a…

A: The Most Probable Number (MPN) is a method for estimating the concentration of viable microorganisms…

Q: Below I have attached the image of gram stain. -We need to find the name of the compound. And after…

A: Gram negative bacteria - The bacteria which are unable to retain crystal violet stain in the process…

Q: - After you are finished, list each step for the Gram staining procedure. - Which of the four steps…

A: NOTE: AS PER OUR GUIDELINES WE NEED TO ONLY ATTEMPT FIRST QUESTION AND ITS PARTS , PLEASE RESUBMIT…

Q: Out of the three gram-positive bacteria (Micrococcus luteus, Staphylococcus aureus, and…

A: Bacteria are small, microscopic and unicellular organisms that can live in variety of environment.…

Q: Can your draw it out and explain it to me please

A: Dilution : The process of lowering a solute's concentration in a solution by increasing the solvent,…

Q: Negative staining is useful for OA.motility purposes OB.visualizing morphology OC.flagella detection…

A: Introduction: Negative staining is a technique for contrasting a thin specimen with an optically…

Q: Staining bacterial capsule can not be accomplished by?

A: Since most standard stains cannot enter the capsule because it is so tightly packed, it is difficult…

Q: which of these tests (i.e gram stain, acid fast stain, endospore, oxidation to fermentation,…

A: Dichotomous Key:A ‘dichotomous key’ is a series of questions about characteristics of an organism,…

Q: What is the purpose of fixing a smear? Mark all that apply: 1. To attach the bacteria to the slide…

A: The staining of microorganisms is very much important that helps us in identifying the morphology…

Q: What test can help you differentiate staphylococci from streptococci? What reagent or media would be…

A: Both are gram positive bacteria.

Q: observe the transparent bacteria under bright field microscope, we use a. Simple staining b. Gram…

A: Microscopy is the technique which is used to magnify  small microorganisms and small specimens . It…

Q: The main of objective of using 13-Line streaking method is to get:

A: Answer: 13-Line streaking method: It is the technique of obtaining pure bacterial culture by…