Additional Differential Staining Procedures: Negative stain, Capsule Stain, Endospore stain, Flagella stain. What is a positive and negative results of this procedures?
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- Which of the following serves as a counterstain for the gram-stain technique? It also happens to be the counterstain for the endospore staining technique.Is heat fixation used on bacterial smears that will undergo a capsule stain or flagella stain procedure? Why or why not?Which two staining techniques employ a chemical (using a reagent, not a physical method) mordant? Gram stain; Acid-fast stain. O Gram stain; Capsule stain. O Capsule stain; Flagella stain. Gram stain; Flagella stain. O Simple stain; Acid-fast stain.
- What staining technique is used here? What is the primary stain, decolorizing agent, and counter stain of this technique? Do we heat fix in here? Describe the organism with the blue color. Describe the organism with the bright pink color. Name the genus of bacteria with bright pink.1) In Gram staining, to what cell structure do the dyes bind? 2) Would it be useful to perform a Gram stain on a mixed culture? Why? 3) In capsule staining, why does the capsules did NOT take in any dye? 4) In endospore staining, what is the purpose of using the steam?what are the positive and negative results of the endospore stain procedure?
- why is it necessary to employ a spore stain rather than rely on diagnosis of sporing from examination of gram stainWhat stain technique must this be? What is used to stain the background? What is used to stain the bacterial cell (in our method)? What structure is the clear (white) layer? Do we heat fix this? Capsule stain, No heat fixation, crystal violet, copper sulfate,With what kind of gram stain do you use heat fixation? What is the purpose of heat fixation? What solution do you use to wash a positive stain? A negative stain?
- Both the Endospore Stain Protocol and Mannitol Salt Agar Media are differential. How can this be so?This part of the staining process helps the primary stain to remain in the cell during decolorization. O 1) Secondary stain O 2) primary stain O 3) counterstain O 4) fixing reagent O 5) mordantDescribe the bacteria observed in different staining procedures. Please refer to the photo - Endospore Stain - Capsule Stain