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- The third micrograph is at the lowest magnification. Identify A (name of the cells; blank 1). Identify B (name of the structures arrows point to; blank 2) - Identify C (name of the structures arrows point to; blank 3) High mag low mag high mag Blank # 1 Blank # 2 Blank # 3Please count the cells in grids 1, 3, 7, and 9. Calculate the cell density (a) and cell viability (b) for this sample. (assume we started with 100 microliters of cells and added 100 microliters of trypan viability blue). Hemacytometer Grid 123 5 6 789 4 Enlarged view O O O 8when preparing the slide. The student used an eyepiece graticule to calculate the size of some of the onion cells. Figure 1 shows the student's eyepiece graticule and stage micrometer. The stage micrometer measures in millimetres. Figure 1 10 20 30 40 Eyepiece graticule 0 mm 2 Stage micrometer Use Figure 1 to calculate the size of one division on the student's eyepiece graticule, in micrometres. Show your working.
- Besides the cheek cells, what other cells might you see on the specimen? Consider both the structure and function of the mouth itself, as well as the procedure by which the cells were collected.How do tissue cells look in comparassion to cheek cells using H&E staining?,.a. How was the specimen prepared for the microscopy technique applied? (for e.g. stained with H&E stain, Gram stain, unstained) b. What is the microscopy technique and magnification used to obtain this image? c. What is the basic principle of image formation using this microscopy technique? d. What can be observed and concluded from the image of the specimen? e. Are there any potential aberrations present in this specimen image? Describe these and how they may affect interpretation of the result.
- With the aid of a diagram, describe the structure of a flexible endoscope capable of carrying out minor surgical procedures.3D dimensionality is a limitation of the compound microscope. Depth of field, DOF, describes dimensionality form top to bottom and can be observed with colored cross threads. Observe the crossed thread slide on low power (4x), then on medium power (10x), then on high power (40x objective magnification). Which crossed fiber is on top? How do you know?Why are appropriate transducer orientation and image standardization important in vascular sonography?
- The benefits of the negative stain include: Mark all that apply: 1. No shrinkage or distortion of cells due to no heat fixing 2. Because the cells don't take up stain they don't get shrunk or distorted 3. One can more accurately determine the cell size and shape because there is no shrinkage or distortion of cells 4. Negatively charged dyes are safer to work with than positively charged dyes 5. One can more accurately determine cell size and shape because the cells stand out against the backgroundDo the bottom diagram Find all th calculations Micrograph width is 0.2mm at 400x Draw a micrograph diagram and calculationsDraw and label 3 cell types at low and high power. State the magnification for each one