Idris has successfully extracted enzymatic proteins from the fish viscera (intestines and stomach). After homogenization and centrifugation, he managed to pool the crude enzyme extract. He is characterizing the enzymes. Please help Idris by answering the following questions: The enzyme has optimum activities at 35oC. With the correct captions for X and Y axes, help me to draw: (a). A temperature profile graph, from 20 to 70oC. (b). Please explain the two ways how temperature can affect the enzyme reaction.

Q: Using the table below, differentiate the effect of two varying pH levels (as indicated by by the…

A: Enzymes are affected by changes in pH. The most favorable pH value - the point where the enzyme is…

Q: Using a pipette, add 2 mL of suspended DEAE SEPHAROSE (anion) resin to a chromatographic column.…

A: DEAE Sepharose matrix is used in Anion exchange column. In the given method power of salt strength…

Q: Can you please help me answer the following question in three paragraphs and in your own words.…

A: Enzymes are substances that enhance the rate of chemical reaction and facilitate the formation of…

Q: The kinetics of an enzyme have been investigated experimentally. Some of the results are given in…

A: Enzymes are highly specialized proteins that have extraordinary catalytic power, greater than that…

Q: Question) In the factory where you work, we can provide you with hydrolysis of lactose, glucose and…

A: “Since you have posted a question with multiple sub-parts, we will solve the first three sub-parts…

Q: What is the interpretation and reaction catalyzed of this GPT enzyme test?.

A: The GPT test, also known as the Alanine Aminotransferase (ALT) test, is a blood test used to measure…

Q: Identify the most cost effective enzyme purification method and provide brief explanations for this…

A: Enzymes need to be purified from cell extract to remove the undesired compounds which helps to…

Q: xplain why each of the following measurements is or is not the result of a Bernoulli trial: (a) The…

A: Bernoulli's trial is generally an experiment that leads to two outcomes for samples are success and…

Q: The table below summarizes the results of three kinetic experiments using the same enzyme and…

A: The double reciprocal curve is used to linearize the Michaelis-Menten curve  to obtain the exact…

Q: When calculating the order of a reaction, the enzyme doesnot appear in the equation. Explain.

A: Enzyme are the biocatalysts which increase the rate of biochemical reaction by decreasing the…

Q: What are the rate constants for the enzyme-catalyzed reaction?

A: Enzymes are a type of proteins that acts as biological catalysts to accelerate the rate of a…

Q: Comment on the ethics surrounding the development of the Pfizer-BioNTech.

A: Vaccines can be described as the weakened or inactive parts of a specific disease-causing organism…

Q: , differentiate the effect of two varying pH levels (as indicated by by the color) to the amylase…

A: It is an enzyme which is produced by salivary glands and it have seen hydrolysis and digestion of…

Q: A16 Compound A is the substrate for two enzymes, E, and E2, involved in the synthe- sis of an amino…

A: The structures of amino acids are simpler than those of antibiotics. It means that the enzyme…

Q: A b

A: You must comprehend the concept of microbial yield (Y) and how it relates to the conversion of…

Q: What are the (2) analytical methods would you suggest if your customer requested proof of purity of…

A: Introduction Enzymes are known as biocatalyst. Chemically enzymes are protein. Enzyme increases the…

Q: A cyclic AMP (CAMP) binding protein was isolated from mammalian cells and characterized by…

A: Given that,The ligand is cAMPThe receptor is cAMP binding protein (CBP)also the concentration of CBP…

Q: An enzymatic reaction was carried out in a batch-operated reactor until exhaustion of the substrate.…

A: Michaelis-Menten equation is used for analyzing the rate of the enzyme-catalyzed reactions in a…

Q: Prepare a standard tube that contains 0.2 mL of 2.5 mM lysine. To this tube, add 0.1 mL of the…

A: Final concentration can be calculated from the formula C1V1=C2V2 Where C1= initial concentration V1=…

Q: Is the following true or false? Biocatalysis is limited to the use of enzymes found naturally in…

A: Biocatalysts are substances used in biochemical reactions. The biocatalysts are used to increase the…

Q: Bradford Assay-Standard Curve 0.800 0.700 0.600 0.500 0.400 0.300 y = 0.0148x - 0.003 R? = 0.9981…

A: Spectrophotometric analysis can be carried out to find the concentration (or amount) of protein in a…

Q: In kool aid chromatography, what factors affect the efficiency of the column? Also discuss the…

A: Kool aid chromatography  The blue dye, is more non-polar than the red dye and is attracted more…

Q: As an intermediate step in making the enzyme solution, you need to prepare Buffer X using the stock…

A: Calculations play an important role in taking the right concentration of solutes. They help to…

Q: Ehermophillic bacterium was isolated from a hot spring at Perak. Bernager Lee is a earcher…

A: A mixture of phenol:chloroform:isoamyl alcohol (25:24:1) is added to promote the partitioning of…

Q: reaction=   Acetaldehyde +NADH +H+  ethanol +NAD+ Describe two procedural changes that would have…

A: The end product of glycolysis is pyruvate. Pyruvate has two possible fates: in presence of oxygen,…

Q: An enzyme with a Km of 3 X 10-4 M was tested at an initial substrate concentration of 10-5 M. In 1…

A: An enzyme with a Km of 3 X 10-4 M was tested at an initial substrate concentration of 10-5 M. In 1…

Q: (a) (b) (c) (d) Identify the type of enzyme immobilization technique. [Kenalpasti jenis teknik…

A: Enzyme immobilization is the process of immobilizing enzymes and thereby prevent their free…

Q: If you wanted to figure out what the optimum temperature is for catalase, how would you set up your…

A: Hi! Thank you for the questions. As you have posted multiple questions, I will be answering the…

Q: A bacterial culture is grown using either octadecane (C18H38) or pentachlorophenol (C6HOCI5)) as the…

A: Bacterial cultures play a crucial role in biodegradation processes, utilizing specific substrates…

Q: Idris has successfully extracted enzymatic proteins from the fish viscera (intestines and stomach).…

A: The first step after crude protein isolation from any sample is the quantification of the total…

Q: You were given the assignment to isolate the enzyme citrate sintetase from a liver homogenate.…

A: The first reaction of the citric acid cycle is a condensation of oxaloacetate (4C) and the…

Q: Which of the following statements regarding size exclusion chromatography is false?     During…

A: Hi! Thank you for the question, as per the honor code, we are allowed to answer the first…

Q: An extract of rat liver was prepared by homogenizing 65 g of tissue in 55 mL of buffer (Fraction 1).…

A: Given details in the problem: Fraction 1 is the initial fraction. Initial specific activity = 11.5…

Q: The pathway shown below is used by a fungus to create Fructose-1,6-diphosphate from glucose. A…

A: The biochemical pathways are regulated by different enzymes that perform a specific chemical…

Q: One of your colleagues has obtained a sample of muscle phosphorylase b that is known to be…

A: Glycogen is storage-type homopolysaccharides that contain two types of glucose polymers: amylose:…

Q: Sydney Brennen isolated Salmonella typhimurium mutants that were implicated in the biosynthesis of…

A: By the use of minimal and deficit medium scientist can characterize different classes of mutants and…

Q: Anan enzyme isolated from a mutant bacterium grown at 20°C works in a test tube at 20°C but not at…

A: Hundreds of metabolites produced by the human gut microbiota accumulate in the bloodstream, where…

Q: The protease used in this procedure functions best at 50°C. Would you expect this enzyme to be…

A: Microorganisms are classified as, psychrophiles, psychrotrophs, mesophiles, thermophiles, and…

Q: 1² L-^ 0.1 + ||| -4-2 |||| 24 [S]-1, (mM-1) 1, The value of Km for the enzyme depicted by curve A is…

A: Km is the Michelle's menton constant which is equal to the substrate concentration at half of the…

Q: A bacterial culture is grown using either octadecane (C18H38) or pentachlorophenol (C6HCl5O) as the…

A: Bacterial culture is a method that allows the multiplication of bacterial cells in or on a culture…

Q: The authors in the abstract given above describe the mechanism for the activation of metallothionein…

A: Protein Phosphatase 2A (PP2A) Binding: Complexes of PP2A PR110 bind to MTF-1, the metal regulating…

Q: An enzyme is mutated such that the KM of the mutated enzyme is lower than the KM of the original…

A: The objective of the question is to understand the effects of a mutation that lowers the KM of an…

Q: A biochemist is trying to determine the type of proteases they have isolated from walrus blubber.…

A: Hi! Thank you for the question. We are authorized to answer one question at a time, since you have…

Q: From the choices provided below, list the reagent(s) in order that will yield the following…

A: Explanation is given in the image.

Q: A genetically modified bacterium was cultivated to produce 1,3-propanediol (1,3-PDO), and the…

A: Oxygen Demand is a measure of the amount of oxidizable substances in a water sample that can lower…