Identify the most cost effective enzyme purification method and provide brief explanations for this method. ▪Repeated freezing and thawing ▪Sonication ▪Homogenization ▪Homogenization ▪Permeabilization lysozyme) by high pressure (French press) by grinding (bead mill) by detergents (e.g. Triton X-100) and/or enzymes (e.g.

Discuss the correct laboratory technique in using the centrifuge for qualitative analysis. Give two tips on its proper use. Explain how calcium ions are confirmed present in a solid salt by using flame test.  Discuss a method to confirm the presence of a) phenol and b) ketone in a test compound. How are proteins confirmed present in a sample? Explain the laboratory process.

Biochemical Tests Principles Culture Media pH Indicators Metabolic End Products Positive Results Carbohydrate Fermentation Test           Indole Test           Methyl-Red Test           Voges-Proskauer Test           Citrate Utilization Test           Catalase Test           Oxidase Test           Coagulase Test           Urease Test           Hydrogen Sulfide Test           Construct a matrix of the various Biochemical Tests by completing this table:

Absorbance is directly proportional to glucose concentration for both routine clinical glucose spectrophotometry methods: glucose oxidase method and the hexokinase method. For each one, name the final product measured in each test reaction, which is proportional to the sample glucose concentration. A.) glucose oxidase: B.) hexokinase: