Human Fbh1 helicase is important in the process of DNA replication. When a muta occurs during the production of Fbh1, the result is a mutant Fbh1 that binds at the replication fork and prevents any helicase protein from attaching to the strand. DNA-polymerase RNA primer DNA-ligase DNA primase Lagging 3 strand 3' 5' 5' 3' Leading strand Helicase Topoisomerase DNA-polymerase Single-strand binding proteins Based on this information and the image shown, what would happen during DNA replication if this mutant helicase were present? Topoisomerase would unwind the DNA and an RNA primer would attach to the DNA molecule and initiate replication. The process would then stop at the blue triang because helicase is needed to separate the strands of DNA. Topoisomerase would unwind the DNA, but then the process would stop at the blue because helicase, the RNA primer, would not be able to attach to the DNA mole and initiate replication. The process would begin at the blue triangle when topoisomerase unwinds the DNA RNA primer attaches to the DNA molecule and initiates replication. DNA polyme would begin the synthesis of each side, but the process would stop because hel would not be present to rejoin the replicated strands. The process could not begin at the blue triangle because helicase is needed to sepa the strands of DNA and initiate the process.

How is DNA copied with leading and lagging strands? Please use these terms in a summary: Helicase  Decide on the leading vs lagging strand locations Primase on leading strand DNA polymerase  Primase on lagging strand  Ligase  Nuclease

Does E. coli chromosomal replication always start at one particular site? What is called? If you were given the DNA sequence of E. coli chromosome, would you be able to identify where E. coli chromosomal replication starts? What is the end of E. coli chromosome replication?

If a mutation occurred that prevented single stranded binding proteins from binding, the result would be: O Replication would not be initiated O DNA would be synthesized on the leading strand, but not the lagging strand Replicating would be initiated, DNA would unwind, RNA primers would be laid down, but DNA polymerase would not bind O The newly synthesized RNA would not be able to leave the DNA template Replication would be initiated, but RNA primers would not anneal to the DNA

DNA polymerase 1 has 5' - 3' polymerase activity. 5'-3' exonuclease activity and 3'-5' exonuclease activity necessary for DNA replication. Mutations in the gene that encodes DNA polymerase 1 may cause the enzyme to lose its function.  Match the consequence of the lost function mutation to the corresponding lost activity.

The umami or savory taste is usually associated with which nutrient?   Question 26 options:   fats   amino acids   carbohydrates   vitamins   Natural gas is treated with a substance that gives it a smell in order to ______.   Question 1 options:   easily detect leaks   prolong its shelf-life   make it less flammable   reduce production costs

Which of the following is not necessary for replication to proceed? O MRNA O RNA primer O DNA polymerase O primase O DNA ligase

As the strands are synthesized in replication, which of the following is true?   the leading strand is synthesized continuously from the 5' to 3' direction while the other strand is synthesized discontinuously in the 3' to 5' direction   both strands are synthesized  in the 5' to 3' direction   the strand that shows the Okazaki fragments is synthesized in the 3'-5' direction.   both strands are synthesized continuously

Which of the following plays a role in repairing thymine dimers by reversing the damage caused by UV light exposure in bacteria? base excision repair photoreactivation enzyme postreplication repair non-homologous end-joining repair DNA polymerase proofreading

Semi-discontinuous replication means that:     DNA synthesis only occurs in the same direction as the replication fork.     the double helix unwinds and each strand serves as a template for a new DNA molecule.     there are Okazaki fragments associated with the leading strand.     DNA synthesis occurs in both a 5’ to 3’ and a 3’ to 5’ direction.     there is both a leading and a lagging strand associated with each replication fork.