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- TOPIC: MAKING A Recombinant DNA modelCan you please check my answer and make sure it is correct. Question: Describe the two roles primers play in PCR. Answer: Primers are short sequences of single stranded DNA that mark both the ends of the target sequence to be amplified during PCR. One primer attaches to the top strand at one end (forward primer) and the other one attaches to the bottom strand at the other end of the segment (reverse primer). They are made specifically to attach to these sections of DNA because the nucleotides of the primers are complementary to the target sequence. Other than marking the starting and end points for PCR, primers are also necessary for the process because without them, DNA polymerase wouldn’t be able to synthesize the complementary strand of DNA because it can only add nucleotides to an existing piece.Method for using PCR to amplify unknown sequences using known sequences by circularizing the template molecule. Reverse Transcriptase PCR Inverse PCR O Hairpin PCR Primer Structures Random Hexamers
- Copy and paste the link below and watch the video on Youtube and Answer the Questionshttps://www.youtube.com/watch?v=g-dNJdOvBM4 Polymerase Chain Reaction Questions: 1. What are the materials used for the polymerase chain reaction? 2. Draw a schematic diagram of the procedure in PCR. 3. Why is it important to design the primers at the start of the laboratory procedure? 4. What are the components of the PCR buffer and what is its pH range? What is the purpose of the buffer? 5. What is the use for magnesium chloride? 6. How much template DNA is added? What is the concentration of the primers? 7. At what temperatures does denaturation, annealing and extension occur? Why are the processes placed in that temperature? 8. In this particular PCR experiment, how many cycles was used? 9. Can this PCR be used on its own to find out if a person has Covid or not on its own? Why or why not?Briefly explain the functions of the four procedures you learned about in this lab: DNA extraction PCR Gel electrophoresis DNA sequencing and analysisWrite the term or phrase that best completes each statement. Use these choices: gel electrophoresis PCR recombinant DNA technology restriction enzymes 16. Scientists use to cut DNA into smaller fragments. 17. A process called. separates DNA fragments by size. 18. During. DNA fragments move to the positive end. > 19. starts with a primer. 20. are bacterial proteins. 21. combines DNA fragments from different sources. 22. A technique called. copies a specific region of DNA. Slencoe/McCiraw-Hill, a division of The McGraw-Hill Companies, Inc.
- Regarding STR markers used in forensic science. Tick all the correct statements: no correct statement the PCR primers used to amplify STRs are located in the repeat units PCR primers to amplify STRs are located on both sides of the repeat units the PCR primers used to amplify STRs are coupled to a fluorochrome which is essential for the detection of amplicons they are absent from the gonosomes the allelic frequencies of STR markers vary according to the ethnicity of the individuals genotyped they are present homogeneously throughout the nuclear genomeDrawback of PCRExplain how and why PCR can be used to amplify DNA. Describe the steps in the process. Be sure to address the role of Taq DNA polymerase.
- Can you please check my answer and see if it is correct. Question: In addition to master mix, what else must be added to each PCR tube? Why? Answer: In addition to master mix, the primers must be added to the PCR tubes. Each tube is also reserved for one individual’s specific section of DNA. So not only do primers needed to be added that are made specifically for the person’s DNA, but the DNA itself from the suspect needs to be added to the PCR tubes (DNA template strand). We also must have a control which contains sterile water, and one PCR tube reserved for DNA from the crime scene.The Lab technician was responsible for evaluating a genetic disorders by PCR. The technician programmed the termocylcler and left for the weekend. After two cycles, a power failure occured and the PCR reaction stopped. One of his colleque realized that and restart to thermocycler again. However, he did not anything know about PCR and asked you what to do: After running PCR products there was no band on the gel. The person decided to change one of the temperatures in the second cycle. Which temperature can be changed and explain why?RECOMBINANT DNA BRIEFLY, DESCRIBE RECOMBINANT DNA AND GIVE ONE CONCRETE EXAMPLE. EVALUATE THE SIGNIFICANCE/PRACTICAL APPLICATIONS OF THIS DNA TECHNOLOGY BY CONSIDERING ETHICAL AND MORAL IMPLICATIONS BEHIND IT. RECOMBINANT DNA EXAMPLE: MODIFIED TRAIT GENE MODIFICATION RECIPIENT ORGANISM FIELDS OF APPLICATION ALSO, WHAT ARE YOUR INSIGHTS? IN 2 TO 3 SENTENCES.