how Gene Targeting Provides a Way toMutagenize Specific Genes?

Q: What are the 5 gene in order on the E. coli tryptophan operon?

A: The trp operon, found in the bacterium Escheichia coli is a group of genes that encode biosynthetic…

Q: Why is sequencing an essential part if generating mutant protein? What could happen if you didn’t…

A: When generating mutant proteins, sequencing plays an important role in the process. It serves…

Q: My Avya mouse is brownish because a) the CpG island with the transposon is unmethylated. b) it was…

A: Epigenetic systems that facilitate gene-environment interactions are involved in the ability of…

Q: Draw the features of a yeast knock out cassette. How does it integrate into the yeast genome…

A: A gene knockout is a genetic technique in which one of an organism's genes is made inoperative.…

Q: Briefly describe two approaches that are used to edit the germline in embryos using CRISPR-Cas9.…

A: The mechanism of CRISPR/Cas-9 genome editing can be generally divided into three steps: recognition,…

Q: Propose a strategy to prevent HIF-alpha signaling in the TME. What do you think would happen in a…

A: The oxygen-sensitive transcriptional activator HIF-1 (hypoxia-inducible factor-1) is an important…

Q: Which of the following statements is not true of both the LacI and TrpR repressor proteins?a) an…

A: The objective of the question is to identify the statement that is not true for both LacI and TrpR…

Q: Outline the steps that occur when piRISCs silence transposable elements by repressing transcription…

A: Piwi-interacting RNA (ribonucleic acid) or piRNA refers to the small non-coding RNA that is…

Q: You have discovered a new bHLH gene and would like to use ChIP-Seq to learn more about it's role in…

A: A basic Helix-Loop-Helix Domain (bHLH) is a very common domain found in proteins that act as…

Q: Compare and contrast how activators, coactivators, repressors,TFIID, and mediator play a role in…

A: Transcription is a process of transcribing the template DNA into complementary mRNA sequence which…

Q: How can these designed nucleases alter the sequence of the genomic DNA?

A: The Common approach to understand gene function in a systematic and unbiased way is including…

Q: What are the advantages of using the T7 polymerase to control expression?

A: The T7 RNA polymerase system offers precise control over gene expression with high specificity,…

Q: Explain the CRISPR-Cas Mechanism for RNA-Guided Destruction of Invading DNA ?

A: CRISPR or clustered regularly interspaced short palindromic repeats denotes a set of sequences of…

Q: How would you modify a GFP gene located in the nucleus in a mammalian cell to translocate to the…

A: A gene is a short stretch of nucleotides present on a chromosome or DNA molecule, that codes for the…

Q: What are single-stranded RNA fragments that associate with a protein complex to induce gene…

A: RNA interference, also termed as RNA silencing, is a biological reaction to double-stranded RNA that…

Q: What are proximal promoter elements ?

A: Gene is the structural and functional unit in the DNA. Genes are composed of nucleotide nitrogenous…

Q: How do activators bind enhancers in the first place if the enhancer DNA is covered with nucleosomes?

A: In the nucleus of each cell in higher organisms, the DNA molecule is packaged into thread-like…

Q: Diagram the mechanism by which CRISPR-cas functions in the immune system of bacteria.

A: CRISPR (clustered regularly interspaced palindromic repeats) is a type of defense mechanism only for…

Q: Why are transposable elements often called genomic parasites?

A: Transposable elements, also known as jumping genes are short segments of DNA, which have special…

Q: Describe how transposable elements alter the genome

A: Transposable element also known as the jumping gene is a DNA sequence that is capable to change its…

Q: How does cAMP and allolactose work to influence the operon?

A: The two proteins involved regulation of lac operon are CRP (catabolite repressor protein) and lac…

Q: Define the purpose of producing many copies of the gene’sencoded protein in a host cell ?

A: In a single gene, information for making specific protein is encoded. Genes are long, consists of…

Q: Explain the evolutionary/logistical advantages of organizing genes into operons?

A: An operon is a cluster of genes that are functionally related and are controlled by a shared…

Q: What is the Use of a promoterless lacZ gene?

A: In prokaryotes, the DNA-binding protein controls the expression and function of genes by controlling…

Q: If want to express the gene from monkey, the PET vector or BACMAN would be best? Briefly explain

A: The decision between PET and BACMAN vectors for expressing a gene from a monkey depends on several…

Q: What is a response element? How do response elements bring about the coordinated expression of…

A: Transcription is regulated by the factors that influence elongation by RNA polymerase and stalling…

Q: Explain Sigma factor recognition sequences?

A: Transcription is a process by which the information a strand of DNA is copied into a new molecule…

Q: Explain what are dispersed core promoters ?

A: Transcription is the process of transferring information from DNA to a single-stranded molecule…

Q: What is the nature of the targeting sequence, and what distinguishes it from other types of…

A: Introduction  In the cell, there is a highly regularized transport mechanism that works for the…

Q: what is the purpose of the neo+ gene that is attached to the Bt gene?

A: Gene is the basic functional and physical unit of heredity. They are made up of DNA and are…

Q: Explain the Structures of repressors?

A: A repressor is a protein that shows a negative effect on gene expression. They are proteins that…

Q: How can these designed nucleases modify the sequence of genomic DNA?

A: A nuclease is a protein that can break the phosphodiester links between nucleotides in nucleic…

Q: What are pseudogenes and how are they produced? Are pseudogenes expressed?

A: The DNA is the genetic material that is passed from one generation to the next generation. It is…

Q: How do the ENCODE data vastly help determine which enhancers regulate which genes?

A: The promoter is present downstream to the transcription direction and helps in the attachment of RNA…