how Gene Targeting Provides a Way toMutagenize Specific Genes?

Q: What are the 5 gene in order on the E. coli tryptophan operon?

A: The trp operon, found in the bacterium Escheichia coli is a group of genes that encode biosynthetic…

Q: By means of a fl owchart, show the possible jumps that a transposon can make. Show the involvement…

A: The transposon is a DNA sequence that can move from one genomic location to another by a…

Q: list of mutgenes caused by transversions?

A: Mutation is any  change or demage  to DNA gene which indirectly alters the forms of proteins being…

Q: My Avya mouse is brownish because a) the CpG island with the transposon is unmethylated. b) it was…

A: Epigenetic systems that facilitate gene-environment interactions are involved in the ability of…

Q: Two bacterial genes are transduced simultaneously. What does this suggest about the location of the…

A: Genetic recombination in bacteria occurs by transformation, conjugation and transduction.

Q: Which of the following statements is not true of both the LacI and TrpR repressor proteins?a) an…

A: The objective of the question is to identify the statement that is not true for both LacI and TrpR…

Q: You have discovered a new bHLH gene and would like to use ChIP-Seq to learn more about it's role in…

A: A basic Helix-Loop-Helix Domain (bHLH) is a very common domain found in proteins that act as…

Q: The cI protein in bacteriophage l (lambda) is a transcriptional regulator - name the promoters in…

A: cI is a transcription inhibitor of bacteriophage Lambda. It is also known as Lambda Repressor and is…

Q: At the molecular level, describe how σ factor recognizes a bacterial promoter. Be specific about the…

A: A sigma factor is a protein needed for the initiation of protein synthesis in bacteria. Sigma factor…

Q: Compare and contrast how activators, coactivators, repressors,TFIID, and mediator play a role in…

A: Transcription is a process of transcribing the template DNA into complementary mRNA sequence which…

Q: Based on the mechanism of gene silencing, what featuresof transposable elements does the RNAi…

A: Genes are the stretch of DNA that code for a polypeptide chain.

Q: Describe how an electrophoretic mobility shift assay is used to determine if a protein binds to DNA…

A: An electrophoretic mobility shift assay (EMSA) is a common affinity electrophoresis technique used…

Q: Q1: What common mechanism is employed by the guide RNA to find its target DNA sequence? Q2: How many…

A: In molecular biology, DNA is called as Deoxyribonucleic acid and RNA is called as Ribonucleic acid.…

Q: horizontal gene transfer Explain how transposable elements facilitate the transfer of genetic…

A: Transposable elements or transposable genetic elements are fragments of DNA that have the ability to…

Q: How Host Cells Take Up and AmplifyRecombinant DNA?

A: Recombinant DNA technology is the joining together of DNA molecules from two different species. The…

Q: When considering gene therapies to treat human diseases, clinical scientists make a distinction…

A: Genes and chromosomes can mutate in either somatic or germinal tissue, and these changes are called…

Q: Why is formation of the replicative form of fX174 necessary inorder to make phage-specific mRNA?

A: DNA is the genetic material in most of the organisms. It is mostly double-stranded but some viruses…

Q: Explain how HOTAIR plays a role in the transcriptional regulationof particular genes.

A: A gene is a sequence of nucleotides in genome that codes for a functioning molecule. There is…

Q: How do activators bind enhancers in the first place if the enhancer DNA is covered with nucleosomes?

A: In the nucleus of each cell in higher organisms, the DNA molecule is packaged into thread-like…

Q: xplain any five of the following terminologies: gene regulation, enhancer or silencer sequences,…

A: Introduction: Genomics is an area within genetics that is associated with the sequencing and…

Q: What is the concept behind RNAI? Explain in terms of how it works.

A: A "nucleic acid" is a linear polymer of nucleotides that is a component of the cell's information…

Q: Identify enhancers with GFP reporters?

A: Enhancers are highly parallel in identification of active sites, and they are very tissue-specific.…

Q: What is a negative inducible operon?

A: Regulated genes can be switched on/off depending on cell's metabolic needs. Operon It is a group of…

Q: What changes can be made to an mRNA transcript to be used in a vaccine in order to change its…

A: mRNA is basically composed of protein encoding ORF, surrounded by 5′ and 3′ UTRs, and a 7-methyl…

Q: Describe how RNA primers are removed in eukaryotes.

A: DNA replication is a process in which two DNA molecules are synthesized from a single DNA molecule.…

Q: describe some ways that a gene can be turned on oroff;

A: Genes are DNA  sequences which forms the basic physical and functional unit of hereditary. Genes…

Q: Describe how transposable elements alter the genome

A: Transposable element also known as the jumping gene is a DNA sequence that is capable to change its…

Q: Explain the function of trp operon in bacteria, the mode of that operon, the context that will turn…

A: A group of genes making up a regulatory or control unit. The unit includes regulator,  operator,…

Q: How does cAMP and allolactose work to influence the operon?

A: The two proteins involved regulation of lac operon are CRP (catabolite repressor protein) and lac…

Q: Explain the evolutionary/logistical advantages of organizing genes into operons?

A: An operon is a cluster of genes that are functionally related and are controlled by a shared…

Q: Explain the information gained about the function of WDR62 from the DNA sequence of the normal human…

A: A neurological condition known as microcephaly is brought on by a mutation, or altered DNA sequence.…

Q: Define gene targeting?

A: Genes are the basic structural and functional units of heredity. They are composed of…

Q: Explain how a gene knockdown reagent, such as RNAI or a morpholino, could interfere with expression…

A: The experimental process by which the expression of one or more genes of organisms is reduced is…

Q: Explain Sigma factor recognition sequences?

A: Transcription is a process by which the information a strand of DNA is copied into a new molecule…

Q: The operator of the lac operon is upstream of the gene that codes for enzymes. What does upstream…

A: Lac operon: The lac operon is a genetic regulatory system found in bacteria that controls the…

Q: How to Find gene-regulatory regions, such as promoters andenhancers ?

A: One of the most difficult tasks facing scientists in the post-genomic age is unravelling the…

Q: Explain what are dispersed core promoters ?

A: Transcription is the process of transferring information from DNA to a single-stranded molecule…

Q: Describe how ES cells are used to generate knockoutmice.

A: Knockout mice is a genetically modified mice in which the researchers have inactivated or knocked…

Q: Does CRISPR CAS9 as a defense in bacteria only work for double stranded viral DNA? or any type of…

A: Scientists can use CRISPR to persistently edit genes in living cells and creatures and, in the…