how Gene Targeting Provides a Way toMutagenize Specific Genes?

What are Focused core promoters ?

Why is it important that NEB express I^q cells do not have endonuclease? What could happen if they did?

A newly identified protein from the  cells of the Panopyra plant on Pandora was shown to inhibit translation of its target genes by binding to the 5’ UTR of the mRNA and preventing ribosome binding.  A possible way this inhibition may be relieved by an sRNA would be:  Group of answer choices a)The sRNA acts as a silencer, suppressing the inhibitory protein and allowing translation to take place. b)The sRNA acts as a decoy, sequestering the inhibitory protein and allowing translation to take place. c)The sRNA acts as a marker, flagging the inhibitory protein for ubiquitination and allowing translation to take place.

Briefly explain how a lambda lysogen can provide immunity against infection by another phage with the same immunity region.

BONUS One benefit of operons in bacteria is that they AYO allow for the bacteria to utilize a wide variety of carbon sources. B) O allow for coordinated gene expression of related genes on one mRNA controlled by one promoter. C) O do not need ligands and regulatory proteins as single genes would. D) O do not need any modification of the proteins after they are expressed.

How is the sgRNA different from certain components of the bacterial defense system described?

you design an ideal promoter for a gene to be highly expressed in E. coli cells? Describe strategies and explain