-H on amylase within which the oint within that DH 7.0, 5. to each C. ter into the e 2 in no Ho 6 4 Using a graduated cylinder, add 25 mL of starch solution to the flask labeled pH 3.0. Record the time and swirl to mix. 5 Two minutes after adding the starch transfer 3.0 mL of the enzyme-substrate mixture to one of the prepared cuvettes and record the absorbance immediately in Table 2. 6 Continue to remove 3.0 mL of the enzyme-substrate solution and place it into a cuvette containing 3 drops of iodine every two minutes for a total of ten minutes. 7 Repeat steps 4-6 for the remaining buffered enzyme solutions. TABLE 2 Effect of pH on the Hydrolysis of Starch Time (Minutes) 3.0 7.0 11.0 0 73 73 qabrlar 73 > 3 73 1.930 >3 2 4 73 73 73 73 مرد
-H on amylase within which the oint within that DH 7.0, 5. to each C. ter into the e 2 in no Ho 6 4 Using a graduated cylinder, add 25 mL of starch solution to the flask labeled pH 3.0. Record the time and swirl to mix. 5 Two minutes after adding the starch transfer 3.0 mL of the enzyme-substrate mixture to one of the prepared cuvettes and record the absorbance immediately in Table 2. 6 Continue to remove 3.0 mL of the enzyme-substrate solution and place it into a cuvette containing 3 drops of iodine every two minutes for a total of ten minutes. 7 Repeat steps 4-6 for the remaining buffered enzyme solutions. TABLE 2 Effect of pH on the Hydrolysis of Starch Time (Minutes) 3.0 7.0 11.0 0 73 73 qabrlar 73 > 3 73 1.930 >3 2 4 73 73 73 73 مرد
Chapter10: Reconstitution Of Powdered Drugs
Section: Chapter Questions
Problem 5SST
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