Go to the NCBI’s website at https://ncbi.nlm.nih.gov On the database dropdown menu, select “Gene” and search for “RB1.” The first entry should be on the Homo sapiens version; click the gene name. Use the information to answer the following:What is the chromosomal location of RB1?Scroll down to the “Expression” data. List 2-3 examples of normal tissues that typically express the RB1 gene. Would you say that RB1 expression is very specific or general within tissues?Scroll down to the “NCBI Reference Sequences (RefSeq)” section. Click on the “GenBank” link under NG_009009.1 RefSeqGene. This will take you to the genomic sequence of RB1. How many nucleotides long is the full-length RB1 gene?Return to the RefSeq section in the Gene Database for RB1 (back click once from where you were for part c). Click on the link under “mRNA and Protein(s)” listed as NM_000321.3. This will take you to the mature mRNA sequence data:How many bases long is the full-length RB1 mRNA transcript?Scroll down to “Features.” Click on “CDS.” This will highlight the coding sequence region of the RB1 mRNA. This is the sequence that will be translated into a protein.At what positions (numbers) does the coding sequence start and stop?How long is the coding sequence?How many amino acids should this encode for? (HINT: does the stop codon encode an amino acid?)Scroll back up to “Features.” As you are scrolling, count the number of “exon” links there are.What is an exon?How many exons make up the RB1 transcript?As you scroll through the “Features” list, observe some of the miscellaneous features (“misc_feature”) of the transcript. These are examples of annotations, which give more information about the sequence than just the order of bases. For example, “misc_feature 916…918” is a “phosphothreonine, by CDK1.” What information can you interpret from this annotation?Go back to the RefSeq (back click once) section in the Gene Database entry for RB1. Click on “NP_000312.2” to access the protein’s amino acid acid sequence information:How many amino acids make up the RB protein? Does this value match your prediction in part d.ii.3?Scroll down to “Features.” List two examples of post-translational modifications that can happen to the RB protein

The RB1 gene (Retinoblastoma 1) is a tumor suppressor gene that is located on chromosome 13. The…

Answered: Go to the NCBI’s website at…

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter12: Genes And Cancer

Section: Chapter Questions

Problem 21QP

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Question

Go to the NCBI’s website at https://ncbi.nlm.nih.gov On the database dropdown menu, select “Gene” and search for “RB1.” The first entry should be on the Homo sapiens version; click the gene name. Use the information to answer the following:
1. What is the chromosomal location of RB1?
2. Scroll down to the “Expression” data. List 2-3 examples of normal tissues that typically express the RB1 gene. Would you say that RB1 expression is very specific or general within tissues?
3. Scroll down to the “NCBI Reference Sequences (RefSeq)” section. Click on the “GenBank” link under NG_009009.1 RefSeqGene. This will take you to the genomic sequence of RB1. How many nucleotides long is the full-length RB1 gene?
4. Return to the RefSeq section in the Gene Database for RB1 (back click once from where you were for part c). Click on the link under “mRNA and Protein(s)” listed as NM_000321.3. This will take you to the mature mRNA sequence data:
  1. How many bases long is the full-length RB1 mRNA transcript?
  2. Scroll down to “Features.” Click on “CDS.” This will highlight the coding sequence region of the RB1 mRNA. This is the sequence that will be translated into a protein.
    1. At what positions (numbers) does the coding sequence start and stop?
    2. How long is the coding sequence?
    3. How many amino acids should this encode for? (HINT: does the stop codon encode an amino acid?)
  3. Scroll back up to “Features.” As you are scrolling, count the number of “exon” links there are.
    1. What is an exon?
    2. How many exons make up the RB1 transcript?
  4. As you scroll through the “Features” list, observe some of the miscellaneous features (“misc_feature”) of the transcript. These are examples of annotations, which give more information about the sequence than just the order of bases. For example, “misc_feature 916…918” is a “phosphothreonine, by CDK1.” What information can you interpret from this annotation?
5. Go back to the RefSeq (back click once) section in the Gene Database entry for RB1. Click on “NP_000312.2” to access the protein’s amino acid acid sequence information:
  1. How many amino acids make up the RB protein? Does this value match your prediction in part d.ii.3?
  2. Scroll down to “Features.” List two examples of post-translational modifications that can happen to the RB protein

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