You have a stock solution of protein that you would like to dilute. How much stock protein do you need to add to 800 µL of buffer to make a 1:5 dilution? 200ul 500ul 100ml 100ul

You want to use a serial dilution to make a 1/50 dilution. The first dilution you make is a 1/5 dilution with a total volume of 1 ml. To make that dilution you would add of your stock of your solution and of your solvent. Then you make a 1/10 dilution and add of your solvent. first dilution and O 0.1 ml, 0.9 ml, 0.2 ml, 0.8 ml O 0.3 ml, 0.7 ml, 0.1 ml, 0.9 ml O 0.2 ml, 0.8 ml, 0.1 ml, 0.9 ml 0.2 ml, 0.8 ml, 0.3 ml, 0.7 ml O None of these answer choices

you are ready to elute your MBP-BAP protein from the column using 1.5mL of TBS + 10mM maltose. Create a buffer from the provided 1x TBS and 500mM maltose stock solution.

Calculate the amount of protein (in mg) in Sample 1 if the measurement at A280 = 0.636, taking into account the dilution factor as per question 6 (100ul) and the total volume of extract as per question 4 (140ml). Give your answer as a number with no decimal places.

how to make 100ml of TBST buffer from 1X TBS (tris buffered saline solution) and 0.1% tween-20. I am just confused how to do the calculations when it is 1X TBS and not 10X TBS. Please show calculations

Briefly describe how you would make 500 mls of a 1X electrophoresis running buffer from a 15X stock solution.

In quantitative determination of protein using spectrophotometer how can I plot the absorbance vs wavelength for the proteins given the transmittance values?