You have a stock solution of protein that you would like to dilute. How much stock protein do you need to add to 800 µL of buffer to make a 1:5 dilution? 200ul 500ul 100ml 100ul

Q: What is the charge of an ionized peptide in the mass spectrometer if its isotopic peaks are 0.20 m/z…

A: Hi! since you haven't mentioned which question to be answered, we will answer one of the question…

Q: How can a mass spectrometer be used to identify the protein/s excised from a 2D gel?

A: Mass spectrometry (MS) is an analytical technique that is used to measure the mass-to-charge ratio…

Q: Below is a trace from an analytical gel-filtration column upon which a single sample containing a…

A: Gel filtration column is used in gel filtration chromatography (GFC) technique. GFC is also known as…

Q: We want to test different concentrations of an antibiotic to see how well it can kill bacteria on a…

A: Introduction:- The Agar dilution of the method is the currently the reference that standard the…

Q: We're attempting to isolate and purify a single protein. Gel filtration chromatography columns allow…

A: Answer: CHROMATOGRAPHY : It is the technique used to isolate the molecules depending upon their size…

Q: draw a gel with samples treated similarly with components below

A: Gel electrophoresis is a separation technique of charge molecules based on their size (molecular…

Q: How do you calculate the amount of ammonium sulfate necessary to get a 20% solution?

A: Ammonium sulfate is the most commonly used for protein precipitation in protein purification.

Q: The order is for Moxam 5 gm IM every 8 hours.  On hand is 10 gram of Moxam with the following…

A: Medication order is the terms used to define the directions for a drug needed to be given to the…

Q: 4 mL of 10% TCA solution was added to 1 mL of serum and after mixing, it was waited for two minutes…

A: Calibration graphs are used to estimate the concentration of unknown sample by plotting it against…

Q: Ideal solvents for fat extraction should: a- penetrate sample particles readily, be in single…

A: Asked: Correct option regarding ideal solvent for fat extraction

Q: You are running a size exclusion column to purify your 25 kDa protein from a lysate mixture. The…

A: Size exclusion column chromatography: As a function of their respective sizes, molecules are…

Q: Can you please do number 1 and show the steps

A: The metric system is an internationally recognised decimalised system of measurement. It is in…

Q: Identify the structure indicated by the letter B. A B C 19

A: The heart is a vital organ responsible for the circulation of blood throughout the body. It has four…

Q: If you have to prepare 4 nM solution of human IgG ( immunoglobuline) which has molecular mass of 150…

A: The number of moles of a solute present in one liter of the solution is called the molarity of that…

Q: An amino acid mixture of phenylalanine, glycine and glutamic acid is to be separated by paper…

A: Paper chromatography is the analytical process by which mixtures of complex compounds such as amino…

Q: Amount of water added (mL) Amount of Stock Solution (mL) Final Concentration A Solution 1 0.…

A: The concentration of unknown solution can be calculated from the formula C1V1= C2V2 Where C1=…

Q: Would it be a good idea to make up a pH 4.0 sodium acetate buffer? Why or why not?

A: 1. Buffer Capacity and Effective pH Range:A buffer is most effective when it is within ±1 pH unit of…

Q: Given 10 gram of vitamin C (E=0.18), we can make ml of isotonic Vitamin C solution.

A: Isotonic solution has the same water concentration and solute concentration as in the cytoplasm of…

Q: what type of gel must be used when the protein size is 2500 Da

A: A type of size exclusion chromatography in which the separation of components is based on the…

Q: Using ultrafiltration, you concentrate a solution of Protein X as follows: Initial volume protein X…

A: Ultrafiltration is a laboratory technique used to remove minute-level impurities from biological…

Q: How should you prepare your running buffer?

A: SDS-PAGE is method of separation of protein based on its size. Running buffer of the SDS-PAGE…

Q: what is the dna concentration suspended in PO4 buffer at ph 7.0 that has an absorbance at 260 nm…

A: DNA concentration of a sample can be determined using spectrophotometry and measuring the absorbance…

Q: There is An order is received for 10 fl oz of a 5% solution. In stock is a 10% solution. How many…

A: Introduction:-  Initial concentration of solution is 25% and we have to make it 5% 300ml solution.…

Q: Hd 12 10 8 6 4 2 0 not text. 0.5 only, no text. only, no text. 1 1.5 NaOH Equivalents 2 You create…

A: Amino acids are biomolecules k that have an amino group, a carboxyl group and a side group linked to…

Q: can you tell what the pKas are from this unknown amino acid based on buffering regions and narrow…

A: pH = If [H+] concentration in a solution is high, the pH will be low and the solution is acidic. If…

Q: Figure 3: ● • . ● KDa ● 97.4 66.2 45.0 ● 31.0 21.5 14.4 S-1 p-1 2.8 2-d S.3 p-3 This figure was…

A: The study of protein behavior under different conditions is a fascinating aspect of molecular…

Q: 10mL of 1x solution from a 2x stock solution.

A: Dilution formula - C1V1 = C2V2 C1 = concentration of stock C2 = final concentration V1 = volume of…

Q: why is dtt added onto the total protein buffers?

A: Buffer is a solution that helps in maintaining the pH during a reaction. All proteins can act as…

Q: a) mentioned the name of simple laboratory method to roughly estimate the concentration of an…

A: Proteins have wide variety of essential function in our body like providing energy, structural…

Q: An amino acid mixture of phenylalanine, glycine and glutamic acid is to be separated by paper…

A: Paper chromatography is the analytical process by which mixtures of complex compounds such as amino…

Q: 2.) An unknown amino acid was subjected to paper chromatography with five amino acid standards in…

A: For Thin layer chromatography, we define Retension factor (Rf) as ratio of distance travelled by…

Q: Ninhydrin test for amino acids or protein requires that sample test solution should be at initially…

A: Proteins are one of the essential macronutrients for a human body. The building blocks of proteins…

Q: In the case of a protein suspension, which of the following statement/s is/are TRUE? I. The smaller…

A: Gel exclusion chromatography is a low resolution isolation technique that makes use of a neat…

Q: You have a 6 M solution of stock A. You do the following serial dilutions: Add 1 ml of stock A to…

A: Dilution is calculated by stock volume to the final volume.  Solution = Stock volume/ (stock volume+…

Q: A chromatography column is used to separate four ami serine, alanine, and histidine. A cylindrical…

A: Chromatography, a group of methods for separating very small quantities of complex mixtures with…

Q: Which of the following chromatographic techniques allows a faster elution of polar proteins? O…

A: Proteins are composed of amino acids, which are bound together by peptide linkage. Amino acids…

Q: Why is pH important for the buffer?   The pH is important to the stability of the gel.     The pH…

A: @ Why is pH important for the buffer?  Answer - pH is important for buffer for processes and/or…

Q: Consider a crude extract with a mixture of the 5 proteins listed below. Protein A 4.5 pl 160…

A: Ion Exchange ChromatographyIon exchange chromatography can be used to separate proteins that have a…

Q: Specify whether each condition would result in increasing or decreasing acidity by dragging each…

A: I. CONCEPTS:   A. According to Arrhenius definition of an acid, it is a species that increases the…

Q: Describe how the following technique work on protein purification? ammonium sulfate precipitation

A: Proteins are organic macromolecules that assist in maintaining the structural and functional…

Q: How do you make 70 ml of a 60% solution??

A: The making of solutions of different concentrations can be achieved with the help of principle…

Q: If I am making 500ml of a solution with 0.625ml ITS, 0.0035ml Linoleic acid, 20ml Nu-serum, 0.75g…

A: A homogeneous mixture of two or more substances is referred to as a solution in chemistry when the…

Q: I had a protein that was pH 5.6. I then used ion exchnage chromatography with the equilibration…

A: Ion exchange chromatography is used for the separation of charged molecules. In this type of…