Gene conferring Strs. - Recipient chromosome Gene conferring StrR. Double-stranded DNA binds to the surface of a competent cell. 2 Single strand enters the cell; the other strand is degraded. 3 The strand integrates into the recipient cell's genome by homologous recombination. The strand it replaced will be degraded. Streptomycin-sensitive daughter cell- Streptomycin-resistant daughter cell After replicating the DNA, the cell divides. Non-transformed cells (Str®) die on streptomycin-containing medium, whereas transformed cells (Str) can multiply. FIGURE 8.19 DNA-Mediated Transformation The donor DNA in this case contains a gene conferring resistance to streptomycin (Str").

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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How would this figure change if double-stranded DNA were incorporated into the donor cell’s chromosome?

Gene conferring Strs.
- Recipient chromosome
Gene
conferring StrR.
Double-stranded DNA binds to the surface of a competent cell.
2
Single strand enters the cell; the other strand is degraded.
3
The strand integrates into the recipient cell's genome by homologous
recombination. The strand it replaced will be degraded.
Streptomycin-sensitive
daughter cell-
Streptomycin-resistant
daughter cell
After replicating the DNA, the cell divides.
Non-transformed cells (Str®) die on streptomycin-containing medium,
whereas transformed cells (Str) can multiply.
FIGURE 8.19 DNA-Mediated Transformation The donor DNA
in this case contains a gene conferring resistance to streptomycin (Str").
Transcribed Image Text:Gene conferring Strs. - Recipient chromosome Gene conferring StrR. Double-stranded DNA binds to the surface of a competent cell. 2 Single strand enters the cell; the other strand is degraded. 3 The strand integrates into the recipient cell's genome by homologous recombination. The strand it replaced will be degraded. Streptomycin-sensitive daughter cell- Streptomycin-resistant daughter cell After replicating the DNA, the cell divides. Non-transformed cells (Str®) die on streptomycin-containing medium, whereas transformed cells (Str) can multiply. FIGURE 8.19 DNA-Mediated Transformation The donor DNA in this case contains a gene conferring resistance to streptomycin (Str").
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