GalK Section/Counterselection When galK is replaced with the gene of interest, these pacteria are able to grow on galactose minimal media.

Σ P UTF-8"Chapter 12 Solutions 202 x 02-xythos.content.blackboardcdn.com/5b19550bob538/10621560?X-Blackboard-Expiration3=D1649052000 DWorksheet.docx Edits will not be automatically saved. aved changes MOu a Garamond 14. B. 三: 三 山 8. When placed in each of the following, indicate if a red blood cell will 1) not change 2) hemolyze 3) crenate 5%(m/v) glucose solution 1%(m/v) glucose solution 0.5%(m/v) NaCl solution 2%(m/v) NaCl solution

Following is the data and notice that it is a terrible idea to culture hMSCs longer than 10 days. You’re strongly Days # cells0 50001 75002 125003 125004 218005 287006 530007 1143008 1653009 19200010 19200011 11680012 8950013 8830014 78300 Part1 You are working for a start-up that is pursuing a clinical trial. The trial involves grafting hMSCs intopatients suffering from interveterbral disc disease using a degradable polymer scaffold. You are going to 3Dprint a porous cylindrical scaffold that is 2 cm in radius and 1 cm in height (matching the dimensions of adegenerated disc). Assume a porosity of 50%. You will fill available volume of the scaffold with hMSCs at adensity of 1 million cells per cm3. Based on the data above, what starting number of cells will you use andhow long will it take you to get enough cells for the trial? Part2The trial is a failure (patients did not report any reduction in back pain). Your team wants to try againusing 85% hMSCs and 15% nucleus pulposus cells .…

Determine the titre of the undiluted phage. 245 Counted plaques. We plated 100 micro Liters (1/10th) of a 10^-8 dilution.

Are the bands indicated in Step 6 (SDS-PAGE) likely visualized by western blot or by a general protein stain (such as Coomassie or silver)?

You first need to create a plasmid. What are the minimal components necessary to develop this plasmid? In addition to these components, please draw the plasmid. In this illustration, I am looking for the components, the direction of transcription (i.e., the direction the genes will be transcribed), and what should be transcribed last? Also, where would you specifically insert the P. falciparum gene in this plasmid, and why are you checking reading frames in your gene? Finally, please name your plasmid using the correct nomenclature too.  You are excited you have this new plasmid; you want to transfect it into P. marinus and provide it as an oral vaccine to laboratory mice. However, even though your supervisor enjoys your enthusiastic attitude, they do not allow you to do this quite yet because all you have is this plasmid. Why doesn’t your supervisor allow you to use the laboratory mouse for this research regarding animal welfare guidelines? Your answer should include the 3 R’s of…

interpret findings. Which one is successful transducted.

An F' cell with a functional copy of the threonine gene on the bacterial chromosome, but with a mutated copy on the plasmid is conjugating with an F-, thr+ cell. Assuming that recombination occurs 1. F' thr+/thr- The GENOTYPE of the Donor Cell after 2. F' thr+/thr+ conjugation. 3. F- thr + The GENOTYPE of the Recipient Cell after conjugation 4. F' thr + The GENOTYPE of the 5. F' thr-/thr+ Donor Cell before conjugation. 6. F- thr-/thr+ The GENOTYPE of the 7. F' thr-/thr- Recipient Cell before conjugation. 8. F- thr-

Genome for C. diphtheriae have about 2,500,000 nucleotides, 87% of them are coding. This ingle circular chromosome contains 2,389 genes from which 2,272 proteins are coded. It does not contain any plasmids. The genome contains Pathogenicity Islands (PAIs), which C. diphtheriae has 13.  What is a PAI and what are their characteristics?

8 of 15 3-6 Insert Plasmid GITAA In this diagram, name the enzyme that converts the DNA substrates on the left into the product on the right. O Terminal transferase O Phosphatase O Topoisomerase O Ligase O Glycosylase