True or false?  wild type fast plants propagate mostly via self pollination   transfected beta-galactosidase requires antibiotics to be expressed properly.   the bradford assay allows you to determine the concentration of proteins produced fromtransfected DNA

Virology 8. A positive sense RNA sequence is given below. Give the correct sequence of the CDNA first strand synthesised by reverse transcriptase. 5'-UUCCGAUAUCGGCACUGAGUA-3' 5'- -3'

Homogenize cells Centrifuge Heat-kill Recover IIIS filtrate IIIS cells spun to bottom of tube Extract carbohydrates, lipids, and proteins IIIS cells in liquid culture medium Treat with ribonuclease Treat with Treat with deoxyribonuclease protease Assay for Transformation IIR cells IIR cells IIR cells IIR cells + IIIS filtrate DNase-treated RNase-treated IIIS filtrate Protease-treated IIIS filtrate IIIS filtrate No transformation Transformation Transformation Transformation occurs occurs occurs occurs IIR cells IIR cells IIR cells Only IIR cells + IIIS cells IIIS cells IIIS cells Conclusion: Active factor is DNA Conclusion: Active factor is not RNA Conclusion: Active factor is Control: IIIS contains not protein active factor E IIIS cells EA IIR cells 8. For each molecule listed, indicate whether they caused a transformation (Yes) or did not cause a transformation (No): a. RNA b. DNA c. Proteins yes d. Carbohydrate e. Lipids

Why is fluorescent in situ hybridization (FISH) a useful tool for identifying micrboes?   DNA is run over a chip, allowing many different gene to be tested     Different probes can show spatial relationships in the cell with other bound probes     None of the other choices     Probes run across a membrane is a natural follow-up to gel electrophoresis     Provides very detailed information about the genetic sequence of the organism

8 of 15 3-6 Insert Plasmid GITAA In this diagram, name the enzyme that converts the DNA substrates on the left into the product on the right. O Terminal transferase O Phosphatase O Topoisomerase O Ligase O Glycosylase

FOR ACADEMIC PURPOSES ONLY - NOT FOR CLINICAL USE Test Details Gene Genotype Phenotype Alleles Tested CYP2C19 *2/*7 Poor Metabolizer *2, *3, *4, *48, *5, *6, *7, *8, *9, *17 CYP2D6 *1/4 Intermediate Metabolizer *2, *3, *4, *4M, "6, *7, *8, *9, *10, *12, *14A, *148, *17, *29, *35, *41 CYP3AS *3/*3 Poor Metabolizer *1D, *2, *3, *38, *3C, "6, *7, "8, *9 CYP3A4 *1/*1 Normal Metabolizer *18, *2, *3, *12, *17, *22 VKORCI -1639G>A G/A Intermediate Warfarin Sensitivity -1639G>A *2, *3 *2, *3, *4, *5, "6, *11 CYP4F2 *1/3 Decreased Function CYP2C9 *1/*1 Normal Metabolizer CYP2B6 *1/*1 Normal Metabolizer *6, *9 Normal Metabolizer - Higher Inducibility CYPIA2 *1F/"1F *1C, *1D, *1F, *1K, *1L, *1V, *1W SLCOIB1 521T>CT/T Normal Function 521T>C, 388A>G CFTR F508del/RS53X Negative Numerous DPYD *1/*1 Normal Metabolizer Numerous ТРМТ *1/*3A *1/1 Intermediate Metabolizer Normal Metabolizer *2, *3A, *38, *3C, *4 *2, *3, *4, *5, "6, *7, *8, *9 NUDT15 UGTIAI *1/*36 Normal Metabolizer *6, *27, *28, *36, *37,…