From a 5% normal saline (NaCl) solution, take 2 ml and dilute it 1:5. Take 5 ml of this solution and dilute it 1:10. A 2:100 dilution is made from this last solution. Determine: a) How many times less concentrated is the 2nd dilution than the original? b) What is the dilution factor in each step of the series and what is the final volume of each dilution? c) How many grams of NaCl will be in 1 ml of the last solution?
From a 5% normal saline (NaCl) solution, take 2 ml and dilute it 1:5. Take 5 ml of this solution and dilute it 1:10. A 2:100 dilution is made from this last solution.
Determine:
a) How many times less concentrated is the 2nd dilution than the original?
b) What is the dilution factor in each step of the series and what is the final volume of each dilution?
c) How many grams of NaCl will be in 1 ml of the last solution?
A serial dilution is a laboratory technique that uses a series of consecutive dilution steps to gradually reduce the concentration of a substance or microorganisms (e.g., cells, bacteria, viruses) in a solution. This method is commonly used in biological research to obtain samples or solutions with lower concentrations for various purposes, particularly in microbiology and molecular biology.
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