f you diluted 200 uL of your culture into 1800 uL of water what is your dilution factor? (Show your work)

Q: Design a serial dilution procedure to achieve a 56-colony count, from a sample with 8.75x105 CFU/mL…

A: Serial dilution is a progression of successive dilutions used to lessen a thick culture of cells to…

Q: My question is: Could you explain steps? (etc. why do we heat or add chemicals like nacl or ammonium…

A: Casein is the major protein component found in milk. It is generally a phosphoprotein which is…

Q: 10ul of the original sample was transferred to 90ul diluent. Then, the mixture was mixed with 100ul…

A: Hemocytometer is blood cell counting device. It is usally cposed of thick glass on which square…

Q: How to count the number of colonies forming units per milliliter (CFUs per ml) of the sample?

A: A viable cell is one that has the ability to divide and form a colony. The colony-forming unit (CFU)…

Q: What is the amount (mL) of pre-culture (108 cell/mL) necessary to inoculate (to add) in a 2-liter…

A: Culture media is a solid or semisolid substance used to support the growth of microorganisms. It is…

Q: how many ml of sterile water injection must be mixed with two 4 ml vials of sterile hypertonic NaCl…

A: Tonicity refers to the ability of a solvent to cause cells to shrink or swell. A semipermeable…

Q: What will you do if you see bubbles forming on the gel after pouring it onto the gel caster/tray?

A: In laboratory, Electrophoresis is a technique for separation of the proteins and nucleic acid. The…

Q: Dilution: 10-

A: intoduction   A Serial weakening is a progression of weakenings, with the weakening variable…

Q: The number of bacterial cells in a culture broth is to be determined by a culture technique. Serial…

A: Colony forming unit refers to the measurement of the number of viable cells present in per ml of the…

Q: How will you prepare a 5% BSA solution? Show all your steps.

A: BSA stands for bovine serum albumin, which is a protein extracted from cows' serum albumin. In lab…

Q: Choose the best combination of depyrogenation methods: 0.45 um filtration +0.22 µm filtration…

A: Introduction Pyrogens are the substance that causes pyrexia( a type of fever). The gram-negative…

Q: A culture medium is comprised of the following components: 0.5% peptone, 0.3% meat extract, 1.5%…

A: In this scenario, we are tasked with preparing a culture medium with specific components, including…

Q: With a plate count average of 297 and a final dilution on the plate of 1:1,000 ; what is the viable…

A: Bacteria are the prokaryotic, unicellular organisms that are sometimes pathogenic in nature. The…

Q: You have 0.05 ml of kombucha that contains microbes at a concentration of 8.2 x 10^6 CFU/ml. You…

A: Creating proper dilutions and working out the colony-forming units or CFU/ ml is one integral part…

Q: An original sample of water containing 4.00 X 106 CFU/mL was diluted by 4 successive 1/10 dilutions.…

A: Number of colonies in the stock suspension = number of colonies plated * 1/ml* Dilution factor  here…

Q: Order: Zosyn 0.02 gm/kg Supply: 5 gm powder vial with directions to add 10 mL of sterile water as…

A: Here we have to calculate how much diluent we need to use to reconstitute Zosyn.

Q: give the respective the TOTAL DILUTION FACTOR of respective serial dilutions. show complete…

A: Introduction: A serial dilution is any dilution in which the concentration decreases by the same…

Q: the step by step process of Nitrate Redution Test using a schematic diagram

A: Nitrate reduction test This test is widely used in microbiology laboratories to determine the…

Q: Use the measurement tool to measure the zone of inhibition for the antibiotic sample in…

A: A).  32.49mm, 32.5mm , 32.5mm     Average - 32.5 mm

Q: If 1.0 mL of a 1:1000 dilution produces 200 colonies on a culture plate, how many bacteria were…

A: Dilution Factor is the factor by which the original solution is diluted. It may be expressed as the…

Q: For the following agar media used, list their significant components and explain how they react.…

A: The sheep blood agar is a popular agar medium. It is a differential medium that includes important…

Q: A laboratory technician performed a series of 10 fold serial  dilutions shown below. he plated 0.1…

A: In this question, we are given colony count from different 10 fold dilutions. The volume plated from…

Q: Where do you expect to see the highest number of CFU on a plate: Select one: a. When pasteurized…

A: The number of colony forming units on the countable plate is multiplied by 1/FDF to get the number…

Q: Compute the cfu/g (lactic acid bacteria and coliforms) of these samples given the following values…

A: Violet Red Bile Agar- is the medium where the coliforms bacteria grow develop sufficiently. The…

Q: A serial dilution was performed for a viable count of bacteria. The original bacterial cuture…

A: Bacteria are unicellular organisms, they grow as colonies. The colonies can be visualized with the…

Q: A nurse reconstituted a vial of 750 mg Cefuroxime Sodium Powder for Injection with 6mL of sterile…

A: Cefuroxime is an antibiotic and is a type of second generation cephalosporin and used for treatment…

Q: Please give explanation for each step other give dislike

A: The term CFU represents "Colony-Forming Unit." It's a metric used in microbiology to calculate how…

Q: You aseptically transfer 1ml of your original liquid culture into 999 ml of sterile water. What is…

A: The dilution factor may be expressed as the ratio of the concentration of stock solution to the…

Q: You place 1 ml of a bacterial broth culture into bottle A containing 99 ml of sterile water and mix.…

A: In serial dilutions, dilutions are multiplied at each step. We can calculate dilution by dividing…

Q: What concentration of ethanol is considered optimum for disinfectant? Why not 100%?

A: Disinfectant refers to the chemical agents that destroy or inactivate microorganisms on an inert…

Q: a sample was diluted by placing a 0.1 mL of dilutent and 1 mL of this dilution was pour-plated.…

A: The CFU/ml in the Original Culture

Q: Determine the number of bacterial cells per milliliter in the original culture. Can you please…

A: For counting the bacterial organism or cell count in the given sample we usually use serial dilution…

Q: Dilute cells from an old culture 1:50 into 200 mL cultures. What volume of the old culture would you…

A: Culture consists of appropriate substances which provides the growth to the desired organisms.

Environmental health and hazards

An organism's environment is the environment in which it lives and has an impact on its well-being. The World Health Organization (WHO) defines environmental health as "those aspects of public health, such as quality of life, that are influenced by physical, chemical, biological, social, and psychosocial components in the environment." Environmental health is concerned with both natural and man-made settings in order to enhance public health. Environmental science, occupational medicine, toxicology, and epidemiology are the key subdisciplines of environmental health.