Order: Zosyn 0.02 gm/kg Supply: 5 gm powder vial with directions to add 10 mL of sterile water as dilutant to create the concentration of 460 mg/mL.        Pt weighs 183 lbs.   How much dilutant will you use to reconstitute Zosyn?

Using 25 uL of a patient's serum, how much diluent would be needed to create a 1:2 dilution? ______ ul

If you transferred 15 mL of an undiluted sample into 35 mL of diluting fluid, what dilution have you created? (Note: provide your answer in decimal format to three decimal places.)

CFU Remember: = Number of Colonies Counted x ml. 1 Dilution Factor # of ml. plated out Treat grams dirt or organic material as follows: 1 gram 1 cubic centimeter (1 cm) =1 mL. %3D CFU V;D;=V,D2 OCD = Volume x Dilution V=Volume D=dilution 1. Calculate the CFU/ml of a urine sample if 49 E. coli colonies were counted on NA when 50 microliters were plated on the NA plate from a 10-6 dilution tube. 2. Plating 0.1 mL of a sample diluted by a factor of 10 produced 43 colonies. What was the original concentration (CFU/ml) in the sample? 3. You have inoculated 100 microliters of a sample diluted by a factor of 103 on a NA plate. After incubation, you count 58 colonies. What was the original concentration (CFU/ml) in the sample?