Explain why micropropagation of banana is a better way of producing planting materials than traditional methodologies. Describe how to carry out micropropagation for this particular crop, including the type of explant to be used and the use of phytohormones or additives in the culture medium needed for each culture stage, along with justifications.
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Explain why micropropagation of banana is a better way of producing planting materials than traditional methodologies. Describe how to carry out micropropagation for this particular crop, including the type of explant to be used and the use of phytohormones or additives in the culture medium needed for each culture stage, along with justifications.
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- 5a)Micropropagation is a beneficial approach in crop propagation as it couldincrease the yield of production besides obtaining disease-free plantingmaterial. Elaborate and discuss this statement.3b)A plant suspension culture was used to study the effect of three types of media on the growth of the culture using batch culture approach. Figure 3.1 shows the growth of the plant suspension cultures using Murashige and Skoog (MS), Gamborg B5 and Vacin and Went media. Comment and conclude on the results obtained. Suggest which is the most suitable medium to maintain the suspension cultures with justification.Explain growth-cycle phases for a batch cultivation. Does Penicillin production occur in log or stationary phase?
- Explain the function of BAP in the preparation of plant tissue culture media.Using a batch culture method, the effects of 3 types of media on the growth of a plant suspension culture was investigated. Figure below has shown the growth of Murashige and Skoog (MS), Gamborg B5, and Vacin and Went media in plant suspension cultures. Discuss on the findings and draw conclusions. With explanation, recommend which medium is best for maintaining suspension culturesIn not more than five lines, outline a method for preparation of tomato cell suspension culture and subculture.
- After performing surface sterilisation on leaves, the leaf explants were cultured on a medium without supplemented with plant growth regulator. Predict THREE possible observations after a week of culture with justifications.1a)A surface sterilisation protocol was carried out to sterilise leaf and stemexplants for evaluating the efficacy of the protocol. All explants were washedthoroughly with distilled water before treating with 50% Clorox for 20minutes. The explants were then rinsed with distilled water before the explants were cultured on plates containing Murashige and Skoog (MS) basal medium. A total of five plates were prepared for each type of explant and each plate consisted of 10 explants. The results recorded after a week are shown in Table. Discuss and conclude the results obtained.What is the major advantage of producing plant by micropropagation?
- In what instances would the pour plate method be more appropriate than the spread plat method?How is the CTSA (Coconut tryptic soyagar) growth medium designed to meet thenutritional need of the bacteria ( psychrotrophic bacteria ) present?Draw a schematic diagram for the preparation of potato sucrose agar plates.