Biochemistry
Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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Two strains of Steptococcus mutans, A and B, use sucrose (table sugar) as a carbon source. The first step in the process of
sucrose utilization is the passage of sucrose through a sucrose transporter protein in the membrane. The characteristics
of the two transport proteins are as follows:
Strain
B
Km
1000 mM
10 mM
Vmax
1000 mmol min
100 mmol min
a) Assuming that the rate of sucrose uptake is the rate limiting step in growth, which strain will grow faster if the
concentration of sucrose is: 10 mM? 100 mM? 1000 mM?
b) One strain was isolated from the soil and the other from the floor at Toscanini's Ice Cream, which was likely to be which?
Why?
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Transcribed Image Text:Two strains of Steptococcus mutans, A and B, use sucrose (table sugar) as a carbon source. The first step in the process of sucrose utilization is the passage of sucrose through a sucrose transporter protein in the membrane. The characteristics of the two transport proteins are as follows: Strain B Km 1000 mM 10 mM Vmax 1000 mmol min 100 mmol min a) Assuming that the rate of sucrose uptake is the rate limiting step in growth, which strain will grow faster if the concentration of sucrose is: 10 mM? 100 mM? 1000 mM? b) One strain was isolated from the soil and the other from the floor at Toscanini's Ice Cream, which was likely to be which? Why?
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