Explain why digestion with EcoRI and Hindlll might give more than one insert fragment with some of the recombinant plasmids

What are the functions of Glacial acetic acid and potassium acetate on plasmid DNA analysis

A newly discovered insect has a genomic DNA that does not contain any repetitive DNA or any intronic regions. The insect’s genomic DNA has been treated with the restriction enzyme EcoRI. One of the fragments (with a length of 4 kb) produced in this manner is isolated and ligated into a pUC19 plasmid vector that has also been treated with EcoRI. This recombinant construct is called plasmid A. Samples of plasmid A, pUC 19, and the total genomic DNA from the insect are digested completely with the restriction enzyme EcoRI. The three digests are loaded into separate wells: plasmid A (lane 1), pUC19 (lane2) and total insect genomic DNA (lane 3) of an agarose gel and subjected to electrophoresis. 2b. Draw what will be seen in each lane if the gel is transferred to a membrane and subjected to Southern blotting and the 4000 base pair genomic DNA fragment (labeled with a fluorescent tag) from the insect is used to probe the blot.

For each of the pLM160 plasmid elements listed below: define the component AND briefly discuss the functional significance of this component for recombineering in Chlamydomonas reinhardtii. a. mNeonGreen-3xFLAG b. KanR2 c. 5’ cloning primer annealing site

draw structure of plasmid

Describe the genetic makeup of a plasmid in an F’ cell, including the types and origins of genetic information contained in this plasmid, and the mechanism by which such a plasmid arises.

Identify mutant genes by plasmid library transformation?

Why does the genomic DNA precipitate upon neutralization, but plasmid DNA does not ?

A plasmid contains genes for ampicillin resistance and chloramphenicol resistance and has single sites for Hind III and Eco RI. When genes are inserted into the Eco RI site, transformants are resistant only to ampicillin. In which region is the site for EcoRI  located? Group of answer choices within the chloramphenicol gene between the ampicillin and chloramphenicol genes can't be determined within the ampicillin gene

Explain why rolling circle replication of plasmids is semi-conservative.