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What are the functions of Glacial acetic acid and potassium acetate on plasmid DNA analysis

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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What are the functions of Glacial acetic acid and potassium acetate on plasmid DNA analysis

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You have two DNA samples. Only one was expected to have plasmid DNA that was isolated from it. Both DNA samples were exposed to two different treatment conditions: untreated, or treated with DNase I. How should the DNase I treatment change how DNA appears on the gel?
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Give a clear handwritten answer...?
Below is shown a plasmid map from a recombinant DNA experiment you have performed. You inserted your gene of interest (labeled gene on the map) into the lac Z reporter gene via a Hind III site. If you digested this recombinant DNA with HindIII what is the size of the DNA fragment(s) you would obtain on a DNA gel electrophoresis? The plasmid alone is 4000 bp. I have attached the image below.
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