Explain the complexities of DNA replication that make the process (a) bidirectional and (b) continuous in one strand and discontinuous in the other.
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- (a) What is the function of helicase in DNA replication?(b) What is the function of DNA polymerase?(c) What are replication forks? Compare and contrast leading and lagging strands. Answer all please(a) Eukaryotic DNA replication is more complex than prokaryotic replication. Give one reason why this should be so. (b) Why might eukaryotic cells need more kinds of DNA polymerases than bacteria?. Indicate the role of each of the following in DNA replication: (a) topoisomerase, (b) helicase, (c) primase,and (d) ligase.
- Describe the functions of the following proteins during DNA replication: (i) Polymerase I (ii) DnaA (iii) TelomeraseDescribe the functions of the following proteins during DNA replication: (i) Polymerase epsilon (ii) Polymerase alpha (iii) Dna A (iv) TelomeraseBelow is a diagram of DNA replication as currently believed to occur in E. coli. Arrows start from numbers and end at specific points. Answer the questions relating to the locations specified by the numbers (1) Which end (5' or 3') of the molecule is here? (2) Which enzyme is probably functioning here to deal with supercoils in the DNA? (3) Which enzyme is probably functioning here to unwind the DNA?
- (a) What will be the problem during DNA replication if the enzyme primase becomes non-functional? (b) In which step of the central dogma is the genetic information of DNA copied into new DNA strands? (c) Which of the following codons is a start codon: GCU, AUG or UGA?Distinguish between (a) unidirectional and bidirectional synthesis, and (b) continuous and discontinuous synthesis of DNA.Describe the three properties of DNA replication.
- Explain how DNA replication is bidirectional and discontinuous in nature?Describe the functions of the following proteins during DNA replication: (i) Polymerase delta (ii) PCNA (iii) TelomeraseOxidative deamination of adenine produces hypoxanthine (the base of inosine), which can base pair with cytosine. (a) If no repair takes place, describe the makeup of the DNA in the two daughter cells following cell division. (b) Describe the makeup of the DNA in the four daughter cells following a second round of cell division.