5. Suppose that you made a mistake when you set up the thermocycler program and omitted the first 2 minutes at 95°C. How would this impact your PCR? 5. Suppose that you made a mistake when you set up the thermocycler program and only programmed for 10 cycles instead of 30 cycles. How would this impact your PCR?
5. Suppose that you made a mistake when you set up the thermocycler program and omitted the first 2 minutes at 95°C. How would this impact your PCR? 5. Suppose that you made a mistake when you set up the thermocycler program and only programmed for 10 cycles instead of 30 cycles. How would this impact your PCR?
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Need help with question 5 and 6
Transcribed Image Text:Exercise II
4. Complete the following table to calculate what volume of primers to add to your PCR master mix:
Per Sample
Master Mix
Added to
Reagent
1X
3.5X
Master Mix?
Water
19.9 ul
69.7 µl
Buffer (10X with Mg2+)
2.5 μι
8.8 μ
Forward Primer (M13F), 10 µM
1 ul
3.5
Reverse Primer (M13R), 10 µM
1 ul
3.5
DNTPS (10 mM each, 40 mM total)
0.5 ul
1.8 μι
Taq Polymerase
0.1 pl
0.4 ul
Total Volume
25 μι
87.5 µl
50
Have your Lab Instructor check your calculations before you add any reagents to your master mix.
W Lab Instructor Initials:
5. Suppose that you made a mistake when you set up the thermocycler program and omitted the
first 2 minutes at 95°C. How would this impact your PCR?
6. Suppose that you made a mistake when you set up the thermocycler program and only
programmed for 10 cycles instead of 30 cycles. How would this impact your PCR?
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