5. Suppose that you made a mistake when you set up the thermocycler program and omitted the first 2 minutes at 95°C. How would this impact your PCR? 5. Suppose that you made a mistake when you set up the thermocycler program and only programmed for 10 cycles instead of 30 cycles. How would this impact your PCR?

The question is attached                         .

BIOTECHNOLGY Date: Name: Instructor: Section/Group:. POST-LAB QUESTIONS 1. In one or two sentences, summarize the technique of gel electrophoresis. 2. How does the process of gel electrophoresis separate DNA fragments? 3. Why is the fact that DNA has a negative charge so important in the gel electrophoresis process? Biotechnology 165

DNa Mapping using Restriction enzymes lab: We will be aliquoting and delivering 5 μl of enzyme to each of the experimental tubes. What would happen if you underloaded the enzyme? i.e. you only delivered 3 or 4 μl ? What would see in your gel results?

Solve 5.

Answer the table below:

If possible please answer all questions based on the gel electrophoresis:  1. What is PCR product size?2. Which reactions were successful/unsuccessful? Which reactions showed clean products? (J1...J6 = jump lines), (N= negative control) & (P= positive control)

Please help solve these. I have tried for over an hour and I am not getting anywhere on any of these

Why is the company Qiagen has more refined DNA extraction steps than a normal Strawberry DNA extraction practical? Summary of Qiagen DNA extraction steps Add ATL buffer and grind with sample. Add 20 microliters of enzyme Proteinase K to degrade protein into a 1.5-2ml microcentrifuge tube. Add 200 microlitres AL lysis buffer, and mix by vortexing for 5–10 seconds, which breaks cell membrane allowing DNA to be released. Incubate the sample at 56 degrees for 10 minutes.  Mix the cell lysate with 200 microlitres ethanol by pipetting it at the side of the microcentrifuge wall so DNA precipitates. The DNA forms a white layer and the remaining liquid is discarded. Pipet the mixture into DNeasy Mini spin column placed in a 2 ml collection tube. Centrifuge for a minute at 8000 rpm. Place the mini spin column into a 2 ml collection tube, add 500 µl Buffer AW1, and centrifuge for 1 min at 8000 rpm. Then add it to a new 2 ml collection tube (provided), add 500 µl Buffer AW1, and centrifuge for 1…

Please solve - no AI answers please. Please refers to image for all detail and explain answers please