Biochemistry
Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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You need linear-linear paper and log-linear paper (also called semi-log paper), for linear-linear,10 squares per inch. For log-linear = semi-log you need at least 3 full cycles.

 

Plot the values below on each type of paper, then select two points marking an approximate doubling of cell density and interpolate back to the ‘x’ axis to determine the time. Indicate the bracketing for your estimate, and state ‘g= ____ hr’

 
 

Time (minutes)          OD420      

            0                      0.091              

           20                     0.092                            

            40                    0.111              

           60                     0.152              

           80                     0.208

         100                     0.303

         120                     0.430

         140                     0.577

         160                     0.732

        180                      0.902

         200                     1.001

         220                     1.084

         240                     1.082

 
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