ELISA link____ to___ in order to create a colorimetric assay
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ELISA link____ to___ in order to create a colorimetric assay
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- You are asked to design an assay to measure the concentration of WonderDrug in various different pharmaceutical preparations using either its UV-visible absorption or its fluorescence emission. Explain when a UV-visible absorption based assay would be the more appropriate and when a fluorescence based assay would be more appropriate.Transillumination is used clinically for the detection of:In an indirect ELISA what does the secondary antibody do (i.e., what purpose does it serve)? Edit View Insert Format Tools Table
- if the ocular lens has a magnification of 20x and the total magnification of the image is 500x. what is the magnification of the objective lens? 480x 10.000x 520x 30x 25x -true or false. the distance the objective lens is from the specimen when the specimen is in focus is called working distance. - in the DNA extraction from fruit laboratory, by what process did cell lysis occur decanting filtering blending mashing measuringWhy is the wavelength of the light that is released in spectrophotometric analysis longer than the wavelength of the light that is used to activate a fluorescent molecule?Copy and paste the link below and watch the video on Youtube https://www.youtube.com/watch?v=8RBs0Ghg_48 Answer the following Questions: 1. What are the chemicals and materials used in gel electrophoresis? 2. Draw a schematic diagram of a gel electrophoresis set-up 3. Describe the procedure in doing a gel electrophoresis experiment. Why is there a need for a leveling bubble/leveler? What is the use of the rubber dam? 4. What is the use of ethidium bromide and why must you wear gloves when you handle it? 5. What makes the DNA fragment move towards the positive plate? 6. What is the purpose of glycerol in the sample buffer? 7. What is the use of a DNA ladder? 8. What will happen when you increase the voltage of the set-up? 9. Can gel electrophoresis be used to separate amino acids? If so, how is it done?
- Why is UV light used in Column chromatography experiment ?How could you utilize fluorescence quenching in the different analytical application? Please answer shorty at your own words. Answer should be specific (4-5 lines) .If a concentrated sample is out of the detection range of a chosen dye-based assay, what can be done?
- Advantages and disadvantages of using nucleic acid detection assay in diagnostic virologyA fellow classmate comes to you to ask your opinion about the following result from this SIM test: (Uploaded Picture). a) Provide a full interpretation and analysis of the results this test. b) Your classmate is not fully convinced about the black coloring seen in this result. Please suggest another type of method that would help confirm this result and what explain what the result should show.What can be the errors and limitations of Gel Electrophoresis practical? Explain in very much detail and also provide some better suggestions.