Electrons carried via NADH Electrons carried via NADH and FADH ELECTRON TRANSPORT CHAIN AND OXIDATIVE PHOSPHORYLATION GLYCOLYSIS KREBS CYCLE Glucose Pyruvate Cytosol Mitochondrion ATP ATP ATP Substrate-level phosphorylation Substrale-level phosphorylation Oxidative phosphorylation nC eg an er sCmey Figure 5. The Process of Cellular Respiration
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- Sonicating a suspension of mitochondria produces submitochondrial particles derived from the innermitochondrial membrane. These membranous vesicles seal inside out, so that the intermembrane space of themitochondria becomes the lumen of the submitochondrial particle. Explain (or diagram) the process of electrontransfer and oxidative phosphorylation in these particles. Assuming all the substrates for oxidativephosphorylation are present in excess, does ATP synthesis increase or decrease with an increase in the pH ofthe fluid in which the submitochondrial particles are suspended? Why?Comparing Glycolysis Entry Points for Sucrose Sucrose can enter glycolysis by either of two routes: Sucrose phosphorylase: Sucrose + Pi fructose + glucose-1-phosphate Invertase: Sucrose + H20 fructose + glucose Would either of these reactions offer Jin advantage over the other In the preparation of hexoses fur entry into glycolysis?pH and in the Chloroplast Proton-Motive Force (Integrates with Chapter 20.) In mitochondria, the membrane potential () contributes relatively more to p (proton-motive force) than does the pH gradient (pH). The reverse is true in chloroplasts. Why do you suppose that the proton-motive force in chloroplasts can depend more on pH than mitochondria can? Why is () less in chloroplasts than in mitochondria?
- EVOLUTION LINK The reactions of glycolysis are identical in all organismsprokaryotes, protists, fungi, plants, and animalsthat obtain energy from glucose catabolism. What does this universality suggest about the evolution of glycolysis?Make a concept map using all of the following terms: GlycolysisOxidation of PyruvateCitric Acid CycleElectron Transport ChainChemiosmosisGlucoseOxidative Phos.Substrate level phosphatePyruvateacetyl-CoACO2OxygenWaterreduced elec. carriers (NADH)oxidized elec. carriers (NAD+)High enerGy Elec.Low enerGy Elec.H+ GradientADP + PiATPHANDOUT C Glucose-6 Phosphate Dehydrogenase and TPI Deficiencies Glucose-6-P GSH + GSH Reduced glutathione 2 H+ NADP+ H₂O₂ Hydrogen peroxide Glucose-6-phosphate dehydrogenase Superoxide radical NADPH + H+ 6-Phosphogluconolactone GSSG Glutathione Oxidized reductase Glutathione peroxidase Aerobic respiration, drugs, fava beans glutathione 2 H₂O Detoxification SOD: superoxide dismutase GPx: glutathione peroxidase HK: hexokinase GSSG and GSH: glutathione, oxidized and reduced forms, respectively CAT: catalase The diagram above gives important details about the HMP shunt. Superoxides/free radicals dis- rupt membrane lipids, causing changes in membrane permeability. The HMP shunt is expressed in liver cells, red blood cells, and in lipid-producing tissues. 1. Discuss how glycolysis is affected in G6PD patients, using the diagrams to explain your response. 2. How does this effect differ from TPI patients? 3. Why are red blood cells, but not the nervous system, affected in G6PD patients?
- Provide an explanation for the intracellular separation offatty acid metabolic processes (i.e., fatty acid biosynthesisin cytoplasm and degradation in mitochondria andperoxisomes).phosphorylation/dephosphorylation 5. Diagram the cascade that regulates glycogen metabolism. Please use key enzyme names and arrows to show how glycogen phosphorylase and glycogen synthase are inversely activated/deactivated.rgy Conversions ite / Unit 2 - The Cell anaeroblc respiration 11) To complete this Venn Diagram, label the following differences and similarities as A (aerobic), B (common to both) or C (anaerobic). with oxygen 2 ATP B convert energy 38 ATP Aerobic Respiration Anaerobic Respiration without oxygen W 12) Matching.
- How many ATP couny after complete oxidation 1 mol glucoseSkeletal muscle cannot synthesize fatty acids, yet it producesthe enzyme acetyl-CoA carboxylase. Explain the role of thisenzymeGlycolysis and Krebs cycle pror TWGPalygXphYW9-zKh7Bt-bdNc75nprw176dBUWOA mResponse 23. Select the situation that will NOT happen if ATP synthase is not functioning. O A. Damage to the chemiosmosis process. B. The number of ATP produced is reduced. C. ATP can be produced through oxidative phosphorylation. D. Accumulation of proton ions in the intercellular membrane. 24. Oxidation of organic compounds that produced carbon dioxide, water and release of free energy causing reduction of the final acceptor molecule: oxygen. Justify the reason. ray bonds than those in