DNA that has been  labeled with 15N is used as the template for replication. Replication is carried using 14N labeled dNTP's. After 3  rounds of replication, what percent of DNA molecules have one strand containing 15N and one strand containing 14N?   A. 50%   B. 75%   C. 25%   D. 100%

Which of the following strands of DNA (assuming it was bound to its complementary strand to create a double-helix structure) would be the least difficult to break apart?   a. 5' - CCGCCGGCATATCCGAT - 3'   b. 5' - CCGCGCGATCGGCGCGT - 3'   c. 5' - AATGAGGCCAATTGACA - 3'   d. 5' - CCACCAGGCACAGCCGA - 3'   e. 5' - AAATTGATATATAGGCA - 3'

1. DNA replication is described as semi-conservative because A. one leading strand and one lagging strand are produced in DNA replication B. all new DNA strands are synthesized continuously C. one new DNA strand is synthesized discontinuously D. DNA replication can never produce DNA molecules which consist of both original DNA stands E. one DNA strand is the template while the complementary strand is not 2. Processivity of a polymerase refers to A. whether the polymerase can process the 5'end of the synthesized molecule B. how long a poly-A tail can be formed C. the efficiency of the removal of introns D. the efficiency in editing E. how long a new strand can be synthesized without dissociation 3. Which of the following processes does NOT hydrolyse ATP or GTP? A. Rho-dependent termination of DNA replication B. Unwinding DNA by helicase C. Complementary base-pairing between A and T D. Formation of fMet-tRNA E. Translocation of ribosome by EF-G 4. An RNA produced from a fragment of DNA…

The replication of chromosomes by eukaryotes occurs in a relatively short period of time because ? a. The eukaryotes have more amount of DNA for replication  b. The eukaryotic replication machinery  is 1000 times faster than prokaryotes c. Each chromosome contains multiple replicons di ba ito? Same eto d. Eukaryotic DNA is always DNA stranded

How many subunits does the E. coli DNA polymerase I have? Select one: O a. 1 O b. 3 О с. 10 O d. 5

DNA polymerase adds nucleotides to Select one: a. the 5′ end, and the leading strand elongates in the 3′ to 5′ direction b. the 3′ end, and the leading strand elongates in the 5′ to 3′ direction c. the 5′ end, and the leading strand elongates in the 5′ to 3′ direction d. the 3′ end, and the leading strand elongates in the 3′ to 5′ direction

Rolling circle replication is important for: plaese explain why you chooice the answer  a. A. conjugation b. B. transformation c. C. transduction d. A and B e. A and C

The leading strand in DNA replication requires only one primer for its synthesis. The lagging strand requires several primers because a. the lagging strand is prepared in short segments b. the lagging strand lacks a topoisomerase c. the leading strand serves as a template for the lagging strand d. All DNA polymerases read from the 5′ end.

On paper, replicate the following segment of DNA:            5’ A T C G G C T A C G T T C A C 3’            3’ T A G C C G A T G C A A G T G 5’a. Show the direction of replication of the new strands and explainwhat causes lagging and leading strands to show different patternsof replication.b. Explain how this is semiconservative replication. Are the newstrands identical to the original segment of DNA?

Which statement regarding DNA replication is false?   A. On a DNA strand that is being synthesized, the 3' end is growing B. On a DNA strand that is being synthesized, the 5' end is growing C. On a DNA strand that is being synthesized, both 3' and 5' ends are  growing D. All of the above  E. None of the above