Q: Compare 2D polyacrylamide gel electrophoresis to LC-MS as a proteomics technique to identify…
A: Polyacrylamide gel is a technique used in organic chemistry, forensic chemistry, genetics, cell…
Q: How can we be sure that the insertion of the therapeutic gene does not harm some other necessary…
A: The Food and Drug Administration still has not approved any human gene therapy product. Current gene…
Q: Describe the methods that are being used to introducegenes into humans for gene therapy.
A: Gene therapy is a clinical field which centers around the usage of the remedial conveyance of…
Q: Summarize the steps required to amplify a given segment of DNA in vivo and in vitro.
A: DNA amplification is a method by which multiple copies of the DNA sequence can be produced. DNA…
Q: Describe the role of restriction enzymes and gel electrophoresis in biotechnology.
A: Biotechnology is the field of biology used to solve many problems of the biological systems.…
Q: What is the purpose of the activity “Transgenic Manipulation?”
A: Transgenic manipulation During the last couple of decades , considerable progress have been made…
Q: Indirect ELISA can detect polygenic gene expression. Rewrite this
A: In polygenic gene inheritance some qualitative characters show discontinuous variation, but a…
Q: What is one stumbling block in AAV gene therapy in some patients?
A: Biotechnology is technology that utilizes biological systems, living organisms or parts of this to…
Q: RT-PCR can determine the amount of mRNA for a particular gene in two different growth conditions.
A: Reverse transcriptase polymerase chain reaction (RT-PCR) is a laboratory technique combining…
Q: Drawbacks of biotechnology in bullets point.
A: Biotechnology is the branch of biology that aims at developing products useful for mankind by using…
Q: Describe the steps involved in the production of a transgenic animal.
A: Transgenic are those which carry and express a foreign gene in their genome transferred either…
Q: What are the considerations for choice of a vector in gene therapy?
A: HIV consists of single-stranded RNA in the replication form that has been transcribed into…
Q: Provide examples of ex vivo and of in vivo gene therapy.
A: Gene therapy is a clinical field which centers around the use of the restorative conveyance of…
Q: Explain gene-knockout technique.
A: According to the question, we have to provide an explanation of the gene knockout technique. So,…
Q: Discuss an example of ex vivo and of in vivo genetherapy.
A: Gene therapy is the science that deals with the insertion of a gene into the defective cells having…
Q: Briefly outline how MLPA probe-sets can simultaneously detect multiple targets using a single set of…
A: Multiplex ligation-dependent probe amplification (MLPA) is an advanced multiplexing technique that…
Q: What is the advantage of using stem cells for genetherapy or gene editing?
A: Gene therapy is the process of replacing the abnormal gene with a normal copy of a gene for the…
Q: What are transgenic Bacteria? Illustrate using any one example?
A: Genetic engineering is the modification of an organism’s genome for a beneficial outcome. Bacteria,…
Q: Make a list of ethical considerations for using gene therapy.
A: Answer: GENE THERAPY = It is the kind of therapy at genetic level where patient is injected with a…
Q: Distinguish between in vivo and ex vivo gene therapy inhumans.
A: The biochemical substances that are carried from the preceding to the succeeding generation are the…
Q: Discuss the Genetically Modified Organism (Benefits and Risks)
A: GMOs are organisms whose genetic material have been altered through the application of genetic…
Q: Compare and contrast ex vivo and in vivo gene therapy as approaches for delivering therapeutic…
A: Viral vectors are used as carriers for normal genes that replace the defective genes within the…
Q: Discuss some advantages and disadvantages of bacteriophage therapyin treating bacterial infections.
A: Bacteriophage is defined as the type of virus that infects bacteria. It is also called as bacterial…
Q: Give three reasons why Salmonella strains are very useful for mutagenesis studies
A: Ans- The standard set of bacterial strains that are used in the mutagenicity testing of…
Q: describe how DNA shuffling and molecular breeding are used to generate hybrid genes and the…
A: DNA shuffling or molecular breeding is an in-vitro molecular method, where mutant genes are created…
Q: Discuss the advantages and disadvantages of using bacterial system instead of Mammalian system to…
A: Bacterial systems biology is the systematic and integrative study of the functions and properties of…
Q: What safety considerations must be taken before CRISPR-Cas isused to edit human embryos to cure…
A: The knowledge of fundamental gene activities and the creation of human disease models in animal…
Q: Explain the rationale and achievable goals behind using a genetic screen(like Heidelberg screen).…
A: Genetic screening such as Heidelberg screening is also called mutagenesis screening. This technique…
Q: Why is it necessary to examine gene-expression profiles, in additionto genome sequences, for…
A: Gene expression profiling is the analysis of the behaviour of thousands of genes at once to…
Q: Outline the steps in making transgenic plants using Agrobacterium tumefaciens.
A: Agrobacterium tumefaciens is a soil bacterium that can integrate its own DNA into the DNA of plant…
Q: During the first successful gene therapy trial in which Ashanti DeSilva was treated for SCID, did…
A: Gene therapy is the process by which defective or non-functional gene can be replaced by a normal…
Q: discuss the biosafety issues involved in the use of genetically engineered microorganism.
A: Biotechnology or genetic engineering where organisms including plants, animals and bacteria or…
Q: Explain about the enhancement gene therapy ?
A: The genes are the primary unit of life. The nucleotide sequence of the genes are responsible for…
Q: Write in detail about the effects of presence as well as absence of MCS and reporter gene in a…
A: MCS (Multiple Cloning Sites ) -- Multiple cloning site is region of D.N.A. with in a plasmid that…
Q: blue-white colony selection method in the recombinant colony screening
A: Blue - white screening for selection recombinat colony :- Blue - white screening is a rapid and…
Q: Describe how ES cells are used to generate knockoutmice.
A: Knockout mice is a genetically modified mice in which the researchers have inactivated or knocked…
Q: Cite three examples of an application of PCR (polymerase chain reaction).
A: Three examples of an application of PCR (polymerase chain reaction) - 1. Amplification of the DNA /…
Q: Briefly present experimental and practical benefits of using PCR in DNA cloning process. Give some…
A: The DNA Cloning is the production of a large number of identical DNA molecules from a single…
Q: what are some of the benefits of producing transgenic crops for farmers and for consumers? List at…
A: A wide range of crop requirement increase day by day. To fulfillment of crop requirement scientists…
Q: Vhat is needed from the cells for PCR?
A: The polymerase chain reaction (PCR) is a technique for replicating a piece of DNA millions of times.…
Q: Among the different application of biotechnology, which do you consider as the most important.…
A: Production of useful products by utilizing biological systems or part of it is known as…
Q: Describe how a transgenic plant can be generated using A. tumefaciens.
A: Transgenic plants are those whose DNA has been altered by genetic engineering. This implies that one…
Q: CRISPR
A: Interestingly, CRISPR-Cas9 could be used to the investigation of treatments of various human…
Q: which method in biotechnology is used to read and understand an oganisum DNA
A: Introduction: a method primarily used to alter the phenotypic of an organism (host) after…
Q: Briefly explain how an antibiotic-resistance gene and the lacZ gene can be used to determine which…
A: Plasmids have been used as cloning vectors in recombinant DNA technology. These plasmids carry genes…
Compare and contrast the advantages and disadvantages of production of genetically modified crps.
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Solved in 2 steps
- describe the blue-white colony selection method in the recombinant colony screening, and present a brief argument for its use in biotechnology labs.What is the purpose of the activity “Transgenic Manipulation?”Why is it necessary to examine gene-expression profiles, in additionto genome sequences, for effective precision medicine?
- discuss the biosafety issues involved in the use of genetically engineered microorganism.Transgenic organisms have foreign DNA incorporated into their genomes that can be expressed to improve the properties of agricultural plants and animals. Provide an application and its potential for global impact.Agrobacterium-mediated plant transformation is a method used to create transgenic plants. Agrobacterium tumefaciens, a soil bacterium works exceptionally well as a natural genetic engineer. Describe briefly the creation of a transgenic plant using A. tumefaciens with illustration.
- Genetic information has become part of our culture and it is difficult to tell the difference between unmodified and genetically modified food sources such as plant and animals. After reading this module’s material regarding vectors in biotechnology, consider the potential for nanotechnology and scientific advancement.Research nanotechnology and its potential use in biotechnology. In one or two paragraphs, explain the potential advantages and disadvantages of nanotechnology in health care, agriculture, or industry and discuss whether you would or would not support further research.Drawbacks of biotechnology in bullets point.In selecting target cells to receive a transferred gene in gene therapy, what factors do you think would have to be taken into account?
- As shown , several medical agents are now commercially produced by genetically engineered microorganisms. Discuss the advantages and disadvantages of making these agents this way.Among the different application of biotechnology, which do you consider as the most important. Defend your answer.RT-PCR can determine the amount of mRNA for a particular gene in two different growth conditions. True False