Discovery of DNA structure A.What are Chargaff’s rules? B. What did Wilkins and Franklin provide that Watson and Crick couldn’t obtain on their own?
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Discovery of DNA structure
A.What are Chargaff’s rules?
B. What did Wilkins and Franklin provide that Watson and Crick couldn’t obtain on their own?
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- Considering DNA sequencing by the Sanger method. It is correct to say that: * A)In the traditional method, radioactively “labeled” primers are used, allowing their visualization in autoradiography. B)In the automated method, a single reaction is performed containing the four “labeled” dideoxynucleotides, each with a different fluorophore. C)In both traditional and automated methods, the fragments are resolved and interpreted according to their ionization state. D)In the automated method, di-deoxynucleotides “labeled” with the same fluorophore are used, thus allowing their interpretation based on graphs of fluorescence emission. E)Sequencing reactions can use mRNA molecules, as long as they have a polyA tail.ule 11 Making Cells – 202 Bb Module 11 Making Cells - 2021 X Bb Take Test: "Lab 11 Homework - X + A sunyocc.open.suny.edu/webapps/assessment/take/launch.jsp?course_assessment_id=_109869_1&course_id=_53817_1&c Question Completion Status: D. Organelle A. Two identical that builds copies of DNA microtubule "highways" to guide DNA B. "staple" that holds DNA copies together C. DNA that is same length and has same instructions; one from mother and one from father Click Save and Submit to save and submit. Click Save All Answers to save all answers. Save All Answers MacBook DD 00 000 F8 F6 F7 F4 F5 F2 F3 F1 * $ % & @ # 2 3 4 * CO < OThree students had a disagreement. Student A claimed a strand of DNA was one large molecule, student B said it was in fact two molecules, and student C argued it was an ordered array of many molecules. Who was correct? Or were they all correct? Explain.
- Give typing answer with explanation and conclusion to all parts Maxim-Gilbert and Sanger Sequencing are two different methods used to sequence DNA. Describe the general techniques of Maxim-Gilbert and Sanger DNA Sequencing. List the advantages and disadvantages of each.Question. What would the forward primer sequence look like if it were intended to bind the area of the DNA template?Compare and contrast Pauling model of DNA to that of Watson & Crick.
- Answer the following parts: A. When performing classical Sanger or "dideoxy" sequencing, you set up 4 parallel reactions per template to be sequenced from a specific primer, with each of the four reactions containing a different dideoxynucleotide, and then the four reactions were run in a separate, adjacent lanes on a gel. Why couldn't you combine all 4 dideoxynucleotides with the primer and the template and do the whole reaction in one tube, and then run the set of fragments produced by the reaction mixture on a single lane in an acrylamide gel? B. When doing automated sequencing, on the other hand, all 4 dideoxynucleotides are added to the same sequencing reaction, and run together in a single capillary gel. What's the difference - why can an automated sequencing reaction be done with all 4 dideoxynucleotides mixed together and all the resulting fragments run together, but not a conventional sequencing reaction?The image below was the basis of Watson and Crick to be able to elucidate the DNA structure. Explain/Discuss the meaning of this image as seen by Watson and Crick and how was it able to support the present-day structure of the Watson and Crick DNA?True or False 1.) The Watson and Crick double stranded DNA structure is always antiparallel. 2.) The Watson and Crick model is a right-handed double helical structure. 3.) The helical turns of the DNA does not only provide spaces for binding with regulatory proteins but they cause the molecule to resemble a spiral staircase. 4.) Adenine, Guanine and Cytosine all have primary amine structures. 5.) In a dsDNA, a pyrimidine in one chain is always paired with a purine on the other chain because of this arrangement, the molecule is 2nm wide along its entire length. Example is A with G or C with T.
- In DNA extracting. What is the purpose of clear shampoo in the DNA extraction buffer?Part C: The Big Picture – In this part, you will use the information from Part A and Part B so answer questions. 1. When you were creating the complimentary DNA strands, what bases (letters) paired together? Were there any bases that could pair with more than one base (letter)? 2. Using the following DNA sequence, ATTACGCATATG, what would the sequence of the complimentary strand be? 3. Could DNA be replicated without the use of enzymes? Explain your answer by giving specific examples. 4. If you replicate on strand of DNA, how many do you end up with? Are the strands identical to the original or different? How do you know? The bases that Paired to)ether Wa sGenetics Attached is a segment of DNA (doublestranded). Answer the following questions about the segment of DNA: How many open reading frames (ORF) are in this sequence? How many amino acids are encoded in all open reading frames in this segment/sequence? Which strand is the template strand for the longest open reading frame?