Did each handwashing treatment result in similar types of organisms growing on the plates? (look at the colonies and gram types) Explain why or why not?

Explain what happens to Gram-positive and Gram-negative bacterial cells at each step of the Gram-staining procedure.

For the picture below, answer the following questions: A. What is the shape of the bacteria? B. What is the arrangement of the bacteria? C. If this slide shows a Gram stain, what is the Gram stain reaction? If this slide does not show a Gram stain, please state that as your answer. ASM MicrobeLibrary.org Smith

You perform several tests on an unidentified bacterial species and get the results below. Based on the test results and the bacterial table, you narrow down the identity to two possible bacteria. Which test should you now do with these bacteria to narrow down the possibilities to just one bacterial species from the bacterial table? (In other words: knowing the results from which test would let you identify these bacteria?) Gram reaction result: Citrate Test (Tube B): O Nitrate reduction test Motility test MSA plate O Glucose fermentation test E

Which of the following can be used to distinguish one colony from another on an agar plate?  Select all that apply. a. Shape b. Color c. Gram stain d. Cell arrangement e. Size f. Elevation g. Margin h. Texture

Why is it essential that the primary stain and the counterstain be contrasting colors in gram-staining?

Which step of the gram stain procedure is most critical?why?

Why does fixation of the positive dye help bacteria stick to the slides?

This is how a hanging-drop slide is prepared. Figure from Macedo, Wikimedia Commons, 2016. 2 3 4 slide cover slip vaseline concavity drop of microbiological culture CA inoculation loop 11. Which slide gives you more information, the hanging drop or the stained slide? Why do you say so? 12. Why might it be valuable to know whether a bacterium is motile?

Attached below is the study about the effects of handwashing and the effectiveness of soap against bacteria. What conclusions can be generated from this result? A C before washing hands 10 second hand wash B D hot water only rinse 30 second hand wash Figure 8: Agar plates with bacteria A) before washing hands B) after rinsing hands in hot water C) After washing hands with soap for 10 seconds and D) after washing hands for 30 seconds. Images show that the number of bacteria on the plate are not greatly reduced after a hot water rinse or 10 second handwash but bacterial levels are reduced after a proper hand wash for 30 seconds.