Describe the whole-genome shotgun strategy

CRISPR/Cas9 can be used in genome editing. Among the following statements, which one is correct?     The CRISPR and the Cas9 parts both recognise the DNA target sequence and then recruit an endonuclease for cutting it     The Cas9 part recognises the DNA target sequence and the CRISPR part cuts it     The CRISPR and the Cas9 parts both recognise and cut the DNA target sequence.     The CRISPR part recognises the DNA target sequence and Cas9 cuts it.

What kind of DNA is actually used to make the spots for analysis for the Vicrochip DNA microassay? From the following which is the best choice To make the spots conserved regions are used from known virus genomes To make the spots a mix of conserved and variable regions are used from knwon virus genomes To make the spots variable regions are used from virus genomes To make the spots conserved regions are used from known virus genomes None of the above

13) The pLacZ cloning vector has an MCS with 10 different palindromes, that is, where you can clone using 10 different enzymes. How many different combinations of enzymes could be used to clone? (This is a measure of the versatility of the vector) a) 2^10 b. 4^3 c. 10^2 d. 3^4 14)One of the first vaccines achieved thanks to the tissue culture technique was that of influenza, True or False? 15) In the context of biotechnology, what does the term clone mean? a) Synonym of Dolly, identical copies of a multicellular organism b. Many copies of some cellular organelle. c. Production of drugs and medicines for diseases, but under the control of ethical standards d. Chimeric molecules (vector and insert), inside a bacteria replicating to form a colony e. Identical copies of proteins from recombinant DNA